Skip to main content
Log in

Development of SCAR marker for simultaneous identification of Miscanthus sacchariflorus, M. sinensis and M. x giganteus

  • Original Paper
  • Published:
Bioprocess and Biosystems Engineering Aims and scope Submit manuscript

Abstract

The sequence-characterized amplified region (SCAR) marker for simultaneous identification of Miscanthus sacchariflorus, Miscanthus sinensis, and Miscanthus x giganteus was developed. In this study, it was attempted for the first time to develop the SCAR marker for detecting the molecular phenotypes among Miscanthus species. Randomly amplified polymorphic DNA technique was applied for this study and one fragment which is unique to M. sacchariflorus was identified and then sequenced. Based on the specific fragment, one SCAR primer pair designated as MS62-5F and MS62-5R was designed to amplify an approximately 1,000 bp DNA fragment within the sequenced region. Diagnostic PCR was performed using the primer pair. Using this SCAR marker, approximately 1,000 bp and 1,200 bp DNA fragments were obtained in M. sacchariflorus and M. sinensis, respectively. Moreover, M. x giganteus was obtained both bands at the same time. The result showed that this SCAR marker can clearly distinguish the M. sacchariflorus, M. sinensis, and M. x giganteus, respectively.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Bauen AW, Dunnett AJ, Richter GM, Dailey AG, Aylott M, Casella E, Taylor G (2010) Modelling supply and demand of bioenergy from short rotation coppice and Miscanthus in the UK. Bioresour Technol 101:8132–8143

    Article  CAS  Google Scholar 

  2. Torney F, Moeller L, Scarpa A, Wang K (2007) Genetic engineering approaches to improve bioethanol production from maize. Curr Opin Biotechnol 18:193–199

    Article  CAS  Google Scholar 

  3. Keshwani DR, Cheng JJ (2008) Switchgrass for bioethanol and other value-added applications: a review. Bioresour Technol 100:1515–1523

    Article  Google Scholar 

  4. Silva CCCM, Ribeiro NFP, Souza MMVM, Aranda DAG (2010) Biodiesel production from soybean oil and methanol using hydrotalcites as catalyst. Fuel Process Technol 91:205–210

    Article  CAS  Google Scholar 

  5. Stephenson AL, Dennis JS, Scott SA (2008) Improving the sustainability of the production of biodiesel from oilseed rape in the UK. Process Saf Environ Protect 86:427–440

    Article  CAS  Google Scholar 

  6. Rayburn AL, Crawford J, Rayburn CM, Juvic JA (2009) Genome size of three Miscanthus species. Plant Mol Biol Rep 27:184–188

    Article  CAS  Google Scholar 

  7. Khasa PD, Dancik BP (1996) Rapid identification of white Engelmann spruce species by RAPD markers. Theor Appl Genet 92:46–52

    Article  CAS  Google Scholar 

  8. Samec P, Nasinec V (1996) The use of RAPD technique for the identification and classification of Pisum sativum L. genotypes. Euphytica 87:229–234

    Article  Google Scholar 

  9. Raina SN, Rani V, Kojima T, Ogihara Y, Singh KP, Deyarumath RM (2001) RAPD and ISSR fingerprints as useful genetic markers for analysis of genetic diversity, varietal identification, and phylogenetic relationships in peanut (Arachis hypogaea) cultivars and wild species. Genome 44:763–772

    CAS  Google Scholar 

  10. Li HB, Wu XQ, Peng HZ, Fu LZ, Wei HL, Wu QQ, Jin QY, Li J (2008) New available SCAR markers: potentially useful in distinguishing a commercial strain of the superior type from other strains of Lentinula edodes in China. Appl Microbiol Biotechnol 81:303–309

    Article  CAS  Google Scholar 

  11. Przyborowski JA, Sulima P (2010) The analysis of genetic diversity of Salix viminalis genotypes as a potential source of biomass by RAPD markers. Ind Crop Prod 31:395–400

    Article  CAS  Google Scholar 

  12. Terashima K, Matsumoto T (2004) Strain typing of shiitake (Lentinula edodes) cultivars by AFLP analysis, focusing on a heat-dried fruiting body. Mycoscience 45:79–82

    Article  CAS  Google Scholar 

  13. Fisher PJ, Gardner RC, Richardson TE (1996) Single locus microsatellites isolated using 5′ anchored PCR. Nucleic Acids Res 24:4369–4371

    Article  CAS  Google Scholar 

  14. Zhang R, Huang C, Zheng S, Zhang J, Ng TB, Jiang R, Zuo X, Wang H (2007) Strain-typing of Lentinula edodes in China with inter simple sequence repeat markers. Appl Microbiol Biotechnol 74:140–145

    Article  CAS  Google Scholar 

  15. Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:6531–6535

    Article  CAS  Google Scholar 

  16. Heun M, Helentjaris T (1993) Inheritance of RAPDs in F1 hybrids of corn. Theor Appl Genet 85:961–968

    Article  CAS  Google Scholar 

  17. Fernandez S, Costa AC, Katsuyama AM, Madeira AMBN, Gruber A (2003) A survey of inter- and intraspecific RAPD markers of Eimeria spp. of the domestic fowl and the development of reliable diagnostic tools. Parasitol Res 89:437–445

    CAS  Google Scholar 

  18. Kim SH, Hamada T (2005) Rapid and reliable method of extracting DNA and RNA from sweetpotato, Ipomoea batatas (L). Lam. Biotechnol Lett 27:1841–1845

    Article  CAS  Google Scholar 

  19. Jaccard P (1908) Nouvelles recherché sur la distribution florale. Bull Soc Vaud Sci Nat 44:223–270

    Google Scholar 

  20. Dnyaneshwar W, Preeti C, Kalpana J, Bhushan P (2006) Development and application of RAPD-SCAR marker for identification of Phyllanthus emblica LINN. Biol Pharm Bull 29:2313–2316

    Article  CAS  Google Scholar 

  21. Su H, Wang L, Ge Y, Feng E, Sun J, Liu L (2008) Development of strain-specific SCAR markers for authentication of Ganoderma lucidum. World J Microbiol Biotechnol 24:1223–1226

    Article  CAS  Google Scholar 

  22. Chou CH, Chiang YC, Chiang TY (2000) Genetic variability and phytogeography of Miscanthus sinensis var. condensatus, an apomictic grass, based on RAPD fingerprints. Can J Bot 78:1262–1268

    CAS  Google Scholar 

  23. Hung KH, Chiang TY, Chiu CT, Hsu TW, Ho CW (2009) Isolation and characterization of microsatellite loci from a potential biofuel plant Miscanthus sinensis (Poaceae). Conserv Genet 10:1377–1380

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by the Rural Department Administration (RDA) grant funded by the Korea Government (No. 12-30-75-PJ007446).

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Bon-Cheol Koo.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kim, J.K., An, G.H., Ahn, SH. et al. Development of SCAR marker for simultaneous identification of Miscanthus sacchariflorus, M. sinensis and M. x giganteus . Bioprocess Biosyst Eng 35, 55–59 (2012). https://doi.org/10.1007/s00449-011-0592-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00449-011-0592-1

Keywords

Navigation