Abstract
Diabetes mellitus (DM) is an important risk factor for erectile dysfunction. Adipose-derived stem cells (ADSCs) are essential for maintaining erectile function but their function is impaired during hyperglycemia. To evaluate the effects of pigment epithelium-derived factor (PEDF)-transfected ADSCs on the restoration of erectile function ADSCs and PEDF-transfected ADSCs were exposed to normal or high glucose levels for 72 h and the effects on cell survival and protein expression were determined. For in vivo studies, rats with streptozocin-induced DM were intravenously injected with ADSCs or PEDF-transfected ADSCs. Two weeks later, the intracavernosal pressure (ICP) and mean arterial pressure (MAP) were measured to assess erectile function, and penile tissues were harvested for further evaluation. PEDF overexpression in ADSCs protected cells against hyperglycemia-induced apoptosis (as determined by a TUNEL assay), increasing the expression of neurotrophic factors and decreasing the expression of inflammatory cytokines and oxidative stress-related proteins (as determined by western blotting and ELISA). In DM rats, transplantation with PEDF-transfected ADSCs effectively restored erectile function, as determined by the ICP/MAP ratio, compared with untreated ADSCs. PEDF overexpression also resulted in higher survival rates and decreased apoptosis of ADSCs. Promotion of neurotrophic factor expression and suppression of inflammatory cytokines and oxidative stress-related proteins were also observed after transplantation of PEDF-transfected ADSCs. Thus, our results demonstrate that transplantation of ADSCs restored erectile function in a rat model of DM, attenuating the negative effects of hyperglycemia. These findings indicate the therapeutic potential of ADSCs for treating erectile dysfunction and the additional benefits of PEDF overexpression.
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References
Adeniyi AF, Adeleye JO, Adeniyi CY (2010) Diabetes, sexual dysfunction and therapeutic exercise: a 20 year review. Curr Diabetes Rev 6:201–206
Albersen M, Fandel TM, Lin G, Wang G, Banie L, Lin CS, Lue TF (2010) Injections of adipose tissue-derived stem cells and stem cell lysate improve recovery of erectile function in a rat model of cavernous nerve injury. J Sex Med 7:3331–3340
Albersen M, Kendirci M, Van der Aa F, Hellstrom WJ, Lue TF, Spees JL (2012) Multipotent stromal cell therapy for cavernous nerve injury-induced erectile dysfunction. J Sex Med 9:385–403
Andersen ML, Guindalini C, Tufik S (2011) Genetics of erectile dysfunction: a review of the interface between sex and molecular biomarkers. J Sex Med 8:3030–3039
Andersson KE (2011) Mechanisms of penile erection and basis for pharmacological treatment of erectile dysfunction. Pharmacol Rev 63:811–859
Angulo J, Wright HM, Cuevas P, Gonzalez-Corrochano R, Fernandez A, Cuevas B, La Fuente JM, Gupta S, Saenz de Tejada I (2010) Nebivolol dilates human penile arteries and reverses erectile dysfunction in diabetic rats through enhancement of nitric oxide signaling. J Sex Med 7:2681–2697
Burnett AL, Lowenstein CJ, Bredt DS, Chang TS, Snyder SH (1992) Nitric oxide: a physiologic mediator of penile erection. Science 257:401–403
Cao W, Tombran-Tink J, Chen W, Mrazek D, Elias R, McGinnis JF (1999) Pigment epithelium-derived factor protects cultured retinal neurons against hydrogen peroxide-induced cell death. J Neurosci Res 57:789–800
Chen X, Li Y, Wang L, Katakowski M, Zhang L, Chen J, Xu Y, Gautam SC, Chopp M (2002) Ischemic rat brain extracts induce human marrow stromal cell growth factor production. Neuropathology 22:275–279
Dawson DW, Volpert OV, Gillis P, Crawford SE, Xu H, Benedict W, Bouck NP (1999) Pigment epithelium-derived factor: a potent inhibitor of angiogenesis. Science 285:245–248
Duh EJ, Yang HS, Suzuma I, Miyagi M, Youngman E, Mori K, Katai M, Yan L, Suzuma K, West K, Davarya S, Tong P, Gehlbach P, Pearlman J, Crabb JW, Aiello LP, Campochiaro PA, Zack DJ (2002) Pigment epithelium-derived factor suppresses ischemia-induced retinal neovascularization and VEGF-induced migration and growth. Invest Ophthalmol Vis Sci 43:821–829
Efimenko A, Starostina E, Kalinina N, Stolzing A (2011) Angiogenic properties of aged adipose derived mesenchymal stem cells after hypoxic conditioning. J Transl Med 9:10
Fedele D (2005) Therapy Insight: sexual and bladder dysfunction associated with diabetes mellitus. Nat Clin Pract Urol 2:282–290, quiz 309
Feng CC, Wang PH, Ding Q, Guan M, Zhang YF, Jiang HW, Wen H, Wu Z (2013) Expression of pigment epithelium-derived factor and tumor necrosis factor-alpha is correlated in bladder tumor and is related to tumor angiogenesis. Urol Oncol 31:241–246
Gur S, Peak TC, Kadowitz PJ, Sikka SC, Hellstrom WJ (2014) Review of erectile dysfunction in diabetic animal models. Curr Diabetes Rev 10:61–73
Hirata H, Kawamoto K, Kikuno N, Kawakami T, Kawakami K, Saini S, Yamamura S, Dahiya R (2009) Restoring erectile function by antioxidant therapy in diabetic rats. J Urol 182:2518–2525
Ho TC, Chen SL, Shih SC, Chang SJ, Yang SL, Hsieh JW, Cheng HC, Chen LJ, Tsao YP (2011) Pigment epithelium-derived factor (PEDF) promotes tumor cell death by inducing macrophage membrane tumor necrosis factor-related apoptosis-inducing ligand (TRAIL). J Biol Chem 286:35943–35954
Kaiho Y, Yamashita S, Arai Y (2013) Optimization of sexual function outcome after radical prostatectomy using phosphodiesterase type 5 inhibitors. Int J Urol 20:285–289
Kurozumi K, Nakamura K, Tamiya T, Kawano Y, Ishii K, Kobune M, Hirai S, Uchida H, Sasaki K, Ito Y, Kato K, Honmou O, Houkin K, Date I, Hamada H (2005) Mesenchymal stem cells that produce neurotrophic factors reduce ischemic damage in the rat middle cerebral artery occlusion model. Mol Ther 11:96–104
Lalu MM, McIntyre L, Pugliese C, Fergusson D, Winston BW, Marshall JC, Granton J, Stewart DJ (2012) Safety of cell therapy with mesenchymal stromal cells (SafeCell): a systematic review and meta-analysis of clinical trials. PLoS ONE 7, e47559
Lee RH, Oh JY, Choi H, Bazhanov N (2011) Therapeutic factors secreted by mesenchymal stromal cells and tissue repair. J Cell Biochem 112:3073–3078
Liu Z, Wang H, Wang Y, Lin Q, Yao A, Cao F, Li D, Zhou J, Duan C, Du Z, Wang C (2012) The influence of chitosan hydrogel on stem cell engraftment, survival and homing in the ischemic myocardial microenvironment. Biomaterials 33:3093–3106
Liu G, Sun X, Bian J, Wu R, Guan X, Ouyang B, Huang Y, Xiao H, Luo D, Atala A, Zhang Y, Deng C (2013) Correction of diabetic erectile dysfunction with adipose derived stem cells modified with the vascular endothelial growth factor gene in a rodent diabetic model. PLoS ONE 8, e72790
Lopushnyan NA, Chitaley K (2012) Genetics of erectile dysfunction. J Urol 188:1676–1683
Lue TF (2000) Erectile dysfunction. N Engl J Med 342:1802–1813
Qiu X, Sun C, Yu W, Lin H, Sun Z, Chen Y, Wang R, Dai Y (2012) Combined strategy of mesenchymal stem cell injection with vascular endothelial growth factor gene therapy for the treatment of diabetes-associated erectile dysfunction. J Androl 33:37–44
Rains JL, Jain SK (2011) Oxidative stress, insulin signaling, and diabetes. Free Radic Biol Med 50:567–575
Shamloul R, Ghanem H (2013) Erectile dysfunction. Lancet 381:153–165
Wang X, Liu C, Li S, Xu Y, Chen P, Liu Y, Ding Q, Wahafu W, Hong B, Yang M (2015) Hypoxia precondition promotes adipose-derived mesenchymal stem cells based repair of diabetic erectile dysfunction via augmenting angiogenesis and neuroprotection. PLoS ONE 10, e0118951
Zhang SX, Wang JJ, Gao G, Shao C, Mott R, Ma JX (2006) Pigment epithelium-derived factor (PEDF) is an endogenous antiinflammatory factor. FASEB J 20:323–325
Zhao Y, Zhang L, Qiao Y, Zhou X, Wu G, Wang L, Peng Y, Dong X, Huang H, Si L, Zhang X, Li J, Wang W, Zhou L, Gao X (2013) Heme oxygenase-1 prevents cardiac dysfunction in streptozotocin-diabetic mice by reducing inflammation, oxidative stress, apoptosis and enhancing autophagy. PLoS ONE 8, e75927
Zhu XF, Zou HD (2012) PEDF in diabetic retinopathy: a protective effect of oxidative stress. J Biomed Biotechnol 2012:580687
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This work was supported by the National Science Foundation for Young Scientists of China (Grant No. 81200504 and 81400755).
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Jun Lu and Zhixiang Xin are co-first authors.
Jun Lu and Zhixiang Xin contributed equally to this work.
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Lu, J., Xin, Z., Zhang, Q. et al. Beneficial effect of PEDF-transfected ADSCs on erectile dysfunction in a streptozotocin-diabetic rat model. Cell Tissue Res 366, 623–637 (2016). https://doi.org/10.1007/s00441-016-2494-7
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DOI: https://doi.org/10.1007/s00441-016-2494-7