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Characterization and phylogenetic analysis of environmental stress-responsive SAP gene family encoding A20/AN1 zinc finger proteins in tomato

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Abstract

Characterization of genes responsive to stress is important for efforts on improving stress tolerance of plants. To address components involved in stress tolerance of tomato (Solanum lycopersicum), a stress-responsive gene family encoding A20/AN1 zinc finger proteins was characterized. In the present study, 13 members of this gene family were cloned from tomato cultivar Pusa Ruby and named as Stress Associated Protein (SAP) genes. Out of 13 genes, 12 have been mapped on their respective chromosomes. Expression of these genes in response to cold, heat, salt, desiccation, wounding, abscisic acid, oxidative and submergence stresses was analysed. All tomato SAP genes were found to be responsive to one or other type of environmental stress. The phylogenetic analysis of these genes, along with their orthologs from Solanaceae species suggests the presence of a common set of SAP genes in the studied Solanaceae species. The present study characterizes a SAP gene family, which encodes A20/AN1 zinc finger containing proteins from tomato for the first time. Genes showing high expression in response to a particular stress can be exploited for improving stress tolerance of tomato and other Solanaceae members.

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Abbreviations

ABA:

Abscisic acid

ExoSAP:

Exonuclease and shrimp alkaline phosphatase

IL:

Introgression line

MS:

Murashige and Skoog

SAP:

Stress-associated protein

TC:

Tentative consensus

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Acknowledgments

This work was financially supported by grants received from the Department of Biotechnology and Defence Research and Development Organization, Government of India. A.U.S. acknowledges CSIR for the fellowship granted during his tenure as a research fellow. We also thank Dr. Dani Zamir for providing resources related to tomato I.L. population and Dr. Shailendra Vyas for critical reading of the manuscript.

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Author notes

  1. Akhilesh K. Tyagi

    Present address: National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi, 110067, India

Authors and Affiliations

  1. Department of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Road, New Delhi, 110021, India

    Amolkumar U. Solanke, Manoj K. Sharma, Akhilesh K. Tyagi & Arun Kumar Sharma

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  1. Amolkumar U. Solanke
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  2. Manoj K. Sharma
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  3. Akhilesh K. Tyagi
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  4. Arun Kumar Sharma
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Correspondence to Arun Kumar Sharma.

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Communicated by K. Shirasu.

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Solanke, A.U., Sharma, M.K., Tyagi, A.K. et al. Characterization and phylogenetic analysis of environmental stress-responsive SAP gene family encoding A20/AN1 zinc finger proteins in tomato. Mol Genet Genomics 282, 153–164 (2009). https://doi.org/10.1007/s00438-009-0455-5

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