Abstract
In order to find natural agents against Dactylogyrus intermedius in goldfish, petroleum ether, chloroform, ethyl acetate, acetone, and methanol extracts of three medicinal plants (Dryopteris crassirhizoma, Kochia scoparia, and Polygala tenuifolia) were screened for antiparasitic properties using in vivo anthelmintic efficacy assay. Among these extracts investigated, methanolic extract of D. crassirhizoma was observed the most effective with EC50 value of 22.97 mg L−1 after 48 h of exposure, which exhibited a 100% efficacy against D. intermedius at 60.00 mg L−1, followed by the methanolic extracts of K. scoparia and P. tenuifolia with EC50 values of 31.28 and 154.79 mg L−1, showing 100% efficacy against D. intermedius at 60.00 and 500.00 mg L−1, respectively. In addition, acute toxicity assay indicated that 48-h LC50 values of methanolic extracts of D. crassirhizoma, K. scoparia, and P. tenuifolia were 4.10-, 2.27-, and 5.00-fold higher than the corresponding EC50. The obtained results demonstrated that methanolic extracts of D. crassirhizoma, K. scoparia, and P. tenuifolia have the potential for the development of novel therapy for the control of D. intermedius in aquaculture.
Similar content being viewed by others
References
Bae K (2000) The medicinal plants of Korea. Kyo-Hak Publishing Company, Seoul, p 25
Bagavan A, Rahuman AA, Kamaraj C, Kaushik NK, Mohanakrishnan D, Sahal D (2010) Antiplasmodial activity of botanical extracts against Plasmodium falciparum. Parasitol Res 108(5):1099–1109
Byung HH, Jeong HP, Myung HP, Yong NH (1985) β-Carboline alkaloids of Polygala tenuifolia. Arch Pharm Res 8(4):243–247
Chang XL, Li W, Koike K, Wu LJ, Nikaido T (2006) Phenolic constituents from the rhizomes of Dryopteris crassirhizoma. Chem Pharm Bull 54:748–750
China Pharmaceutical University et al (1993) Traditional Chinese medicine Cihai, vol I. Chinese Medical Technology Publishing House, Beijing, pp 1937–1939
Dove A, Ernst I (1998) Concurrent invaders—four exotic species of Monogenea now established on exotic freshwater fishes in Australia. Int J Parasitol 28:1755–1764
Finney DJ (1971) Probit analysis, 3rd edn. Cambridge University Press, Cambridge
Gao ZP, Lu WR, Li GF (2002) Screening of the antimalarial parts of the extract of Guanzhong (the rhizome of Dryopteris crassirhizoma). Journal of Beijing University of Traditional Chinese Medicine 25(4):46–46
Goven B, Gilbert J, Gratzek J (1980) Apparent drug resistance to the organophosphate dimethyl (2, 2, 2-trichloro-1-hydroxyethyl) phosphonate by monogenetic trematodes. J Wildlife Dis 16(3):343–346
Ikeya Y, Sugama K, Okada M, Mitsuhashi H (1991) Two xanthones from Polygala tenuifolia. Phytochemistry 30:2061–2065
İsmail KIR, Selda TÖ (2007) Helminth infections in common carp, Cyprinus carpio L., 1758 (Cyprinidae) from Kovada Lake (Turkey). Turk Parazitol Derg 31:232–236, in German with English abstract
Ito H, Taniguchi H, Kita T, Matsuki Y, Tachikawa E, Fujita T (1977) Xanthones and a cinnamic acid derivative from Polygala tenuifolia. Phytochemistry 16:1614–1616
Klinger R, Floyd RF (2002) Introduction to freshwater fish parasites. Document CIR716. Institute of Food and Agricultural Science. University of Florida, Florida
Lee HB, Kim JC, Lee SM (2009) Antibacterial activity of two phloroglucinols, flavaspidic acids AB and PB, from Dryopteris crassirhizoma. Arch Pharm Res 32(5):655–659
Lee JS, Miyashiro H, Nakamura N, Hattori M (2008) Two new triterpenes from the rhizome of Dryopteris crassirhizoma, and inhibitory activities of its constituents on human immunodeficiency virus-1 protease. Chem Pharm Bull 56(5):711–714
Marshall CJ (1999) Use of Supaverm® for the treatment of monogenean infestation in koi carp (Cyprinus carpio). Fish Veterinary J 4:33–37
Matsuda H, Dai Y, Ido Y, Ko S, Yoshikawa M, Kubo M (1997a) Studies on kochiae fructus III. Antinociceptive and antiinflammatory effects of 70% ethanol extract and its component, momordin Ic from dried fruits of Kochia scoparia L. Biol Pharm Bull 20:1086–1091
Matsuda H, Dai Y, Ido Y, Ko S, Yoshikawa M, Kubo M (1997b) Studies on kochiae fructus IV. Anti-allergic effects of 70% ethanol extract and its component, momordin Ic from dried fruits of Kochia scoparia L. Biol Pharm Bull 20:1165–1170
Min BS, Tomiyama M, Ma CM, Nakamura N, Hattori M (2001) Kaempferol acetylrhamnosides from the rhizome of Dryopteris crassirhizoma and their inhibitory effects on three different activities of human immunodeficiency virus-1 reverse transcriptase. Chem Pharm Bull 49:546–550
Nagai T, Suzuki Y, Kiyohara H, Susa E, Kato T, Nagamine T, Hagiwara Y, Tamura SI, Yabe T (2001) Onjisaponins, from the root of Polygala tenuifolia Willdenow, as effective adjuvants for nasal influenza and diphtheria–pertussis–tetanus vaccines. Vaccine 19:4824–4834
Noro Y, Okuda K (1973) Dryocrassin: a new acylphloroglucinol from Dryopteris crassirhizoma. Phytochemistry 12:1491–1492
Prakash A, Rao J (1997) Botanical pesticides in agriculture. CRC Lewis, New York, p XIII
Prabakar K, Jebanesan A (2004) Larvicidal efficacy of some cucurbitacious plant leaf extracts against Culex quinquefasciatus (Say). Bioresour Technol 95(1):113–114
Rahuman AA, Gopalakrishnan G, Venkatesan P, Geetha K (2008) Larvicidal activity of some Euphorbiaceae plant extracts against Aedes aegypti and Culex quinquefasciatus (Diptera: Culicidae). Parasitol Res 102:867–873
Reed PA, Francis-Floyd R, Klinger RC (2009) FA28/FA033: Monogenean parasites of fish. EDIS—Electronic Data Information Source—UF/IFAS Extension. University of Florida. http://edis.ifas.ufl.edu/FA033. Accessed 17 May 2009
Sakuma S, Shoji J (1981a) Studies on the constituents of the root of Polygalae tenuifolia Willdenow. Chem Pharm Bull 29:2431–2441
Sakuma S, Shoji J (1981b) Studies on the constituents of the root of Polygalae tenuifolia Willdenow. Chem Pharm Bull 30:810–821
Schmahl G, Mehlhorn H (1985) Treatment of fish parasites. 1. Praziquantel effective against Monogenea (Dactylogyrus vastator, Dactylogyrus extensus, Diplozoon paradoxum). Z Parasitenk 71:727–737
Schmahl G, Mehlhorn H, Haberkorn A (1988) Sym. Triazinone (toltrazuril) effective against fish-parasitizing Monogenea. Parasitol Res 75(1):67–68
Shin KM, Kim YH, Park WS, Kang I, Ha J, Choi JW, Park HJ, Lee KT (2004) Inhibition of methanol extract from the fruits of Kochia scoparia on lipopolysaccharide-induced nitric oxide, prostagladin E2, and tumor necrosis factor-a production from murine macrophage RAW 264.7 cells. Biol Pharm Bull 27(4):538–543
Shiojima K, Arai Y, Ageta H (1990) Seasonal fluctuation of triterpenoid constituents from dried leaflets of Dryopteris crassirhizoma. Phytochemistry 29:1079–1082
Takiura K, Honda S (1964) Sugar components of the root of Polygala tenuifolia. Yakugaku Zasshi 84:1223–1224
The Pharmacopoeia Commission of People's Republic of China (2005) The pharmacopoeia of the People's Republic of China, vol. I. Chemical Industry Publishing House, Beijing, p 231
Topić PN, Hacmanjek M, Teskeredžić E (2001) Health status of rudd (Scardinius erythrophthalmus hesperidicus H.) in Lake Vrana on the Island of Cres, Croatia. J Appl Ichthyol 17:43–45
Treves-Brown KM (1999) Availability of medicines for fish. Fish Vet J 4:40–55
Wang GX, Zhou Z, Cheng C, Yao JY, Yang ZW (2008) Osthol and isopimpinellin from Fructus cnidii for the control of Dactylogyrus intermedius in Carassius auratus. Vet Parasitol 158:144–151
Wang GX, Han J, Feng TT, Li FY, Zhu B (2009) Bioassay-guided isolation and identification of active compounds from Fructus arctii against Dactylogyrus intermedius (Monogenea) in goldfish (Carassius auratus). Parasitol Res 106:247–255
Wang GX, Jiang DX, Li J, Han J, Liu YT, Liu XL (2010a) Anthelmintic activity of steroidal saponins from Dioscorea zingiberensis C. H. Wright against Dactylogyrus intermedius (Monogenea) in goldfish (Carassius auratus). Parasitol Res 107:1365–1371
Wang GX, Han J, Zhao LW, Jiang DX, Liu YT, Liu XL (2010b) Anthelmintic activity of steroidal saponins from Paris polyphylla. Phytomedicine 17:1102–1105
Woo PTK, David W, Bruno LH, Susan L (2002) Diseases and disorders of finfish in cage culture. CABI, Malaysia
Acknowledgments
This work was supported by the National High Technology Research and Development Program of China (863 Program) (no. 2011AA10A216) and National Natural Science Foundation of China (no. 31072242).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Lu, C., Zhang, HY., Ji, J. et al. In vivo anthelmintic activity of Dryopteris crassirhizoma, Kochia scoparia, and Polygala tenuifolia against Dactylogyrus intermedius (Monogenea) in goldfish (Carassius auratus). Parasitol Res 110, 1085–1090 (2012). https://doi.org/10.1007/s00436-011-2592-0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00436-011-2592-0