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Neospora spp. and Toxoplasma gondii antibodies in horses in the Czech Republic

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Abstract

During January 2007, blood samples were collected from 552 healthy horses from nine different regions of the Czech Republic. Sera were tested for serum antibodies to Neospora caninum by a competitive-inhibition enzyme-linked immunosorbent assay and confirmed by an indirect fluorescent antibody test. The same samples were tested for serum antibodies against Toxoplasma gondii by a latex agglutination test. In total, 131 of 552 (24%) horses reacted positively for Neospora antibodies in competitive-inhibition enzyme-linked immunosorbent assay; seven of them had ≥50% of inhibition. Samples were confirmed in indirect fluorescence test, and only two samples were positive with final titres 50 and 100, while others were negative. Antibodies against T. gondii were found in 125 (23%) horses. This is the first serologic survey for Neospora spp. antibodies performed on horses in the Czech Republic.

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Acknowledgements

This study was supported by the Ministry of Education, Youth and Sports of the Czech Republic (Grant No. MSM6215712402).

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Authors and Affiliations

  1. Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Palackého 1/3, 612 42, Brno, Czech Republic

    Eva Bártová, Michaela Syrová & Ivan Literák

  2. Department of Virology and Serology, State Veterinary Institute Prague, Sídlištní 136/24, 165 03, Prague 6, Czech Republic

    Kamil Sedlák

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  1. Eva Bártová
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  2. Kamil Sedlák
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  4. Ivan Literák
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Correspondence to Eva Bártová.

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Bártová, E., Sedlák, K., Syrová, M. et al. Neospora spp. and Toxoplasma gondii antibodies in horses in the Czech Republic. Parasitol Res 107, 783–785 (2010). https://doi.org/10.1007/s00436-010-1929-4

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  • DOI: https://doi.org/10.1007/s00436-010-1929-4

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