Abstract
The present paper reports the ability of egg-propagated gametocyte vaccine to induce protective immunity against Eimeria tenella infections in offspring chicks. For this purpose, breeding hens were immunized with adjuvanted gametocyte vaccine orally, being the best vaccine in our previous studies. Eggs collected 2 weeks after the boosting dose were allowed to hatch. Upon challenge with sporulated oocysts of E. tenella (local isolates) to 7-day-old chicks from immunized hens showed more than 70% reduction in oocyst production compared with chicks from control hens. Furthermore, no mortality was recorded in immunized chickens, while four out of five control chickens died due to coccidiosis. These results suggested that maternal immunization with egg-propagated gametocyte vaccine can control E. tenella infections in offspring chicks.
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References
Akhtar M, Ayaz S, Hayat CS, Ashfaque M (1998) Immune response of sonicated coccidial oocyst in chickens. Pak J Biol Sci 1:389–391
Akhtar M, Ayaz MM, Hayat CS, Ashfaque M, Hussain I (2002) Adaptation of Eimeria tenella sporozoites (local isolate) in the embryonated hen’s eggs. Pak Vet J 22:40–41
Akhtar M, Hafeez MA, Javed MT, Hussain I (2006) Immunogenic characterization of egg adapted gametocytes of Eimera tenella. Parasitol Res (in press). DOI 10.1007/s00436-006-0160-9
Ayaz MM (2003) Development of egg adapted vaccine (local isolate) against coccidiosis in poultry. Ph.D. thesis, University of Agriculture, Faisalabad, Pakistan
Hafeez MA, Akhtar M, Hussain I (2006) Protective effect of egg propagated Eimeria tenella (local isolates) gametocytes as vaccine(s) against mixed species of coccidian in chickens. Parasitol Res 98:539–544
Karim MJ, Basak SC, Trees AJ (1996) Characterization and immunoprotective properties of monoclonal antibodies against major oocyst wall protein of Eimeria tenella. Infect Immun 64:1227–1232
Laxer MA, Healey MC, Youssef NN (1987) Production of monoclonal antibodies specific for Eimeria tenella microgametocytes. J Parasitol 73:611–616
Ryley JF, Meade R, Ifazalburst J, Robinson TE (1976) Methods in coccidiosis research: separation of oocyst from faeces. Parasitology 73:311–326
Speer CA, Hammond DM, Mahrt JL, Roberts WL (1973) Structure of the oocyst and sporocyst walls and excystation of sporozoites of Isospora canis. J Parasitol 59:35–40
Wallach M, Pillmemmer G, Yarus S, Halabi A, Pugatsch T, Mencher D (1990) Passive immunization of chickens against Eimeria maxima infection with monoclonal antibodies developed against a gametocyte antigen. Infect Immun 58:557–562
Wallach MA, Halabi G, Pillemer O, Sar-Shalom D, Mencher M, Gilad U, Bendheim HD, Danforth C, Augustine PC (1992) Maternal immunization with gametocyte antigens as a means of providing protective immunity against Eimeria maxima in chickens. Infect Immun 60:2036–2039
Wallach M, Smith NC, Petracca M, Miller CM, Eckert J, Braun R (1995) Eimeria maxima gametocyte antigens: potential use in a subunit maternal vaccine against coccidiosis in chickens. Vaccine 13:347–354
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The funds for this project was sponsored by Higher Education Commission, Islamabad, Government of Pakistan under Merit Scholarships Scheme.
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Hafeez, M.A., Akhtar, M., Javed, M.T. et al. Maternal immunization by egg propagated gametocyte vaccine to control Eimeria tenella infections in newly hatched chicks. Parasitol Res 100, 1139–1141 (2007). https://doi.org/10.1007/s00436-006-0382-x
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DOI: https://doi.org/10.1007/s00436-006-0382-x