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The monoclonal anti-BCL10 antibody (clone 331.1) is a sensitive and specific marker of pancreatic acinar cell carcinoma and pancreatic metaplasia

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Abstract

Acinar cell carcinoma (ACC) is a rare pancreatic cancer which may be difficult to distinguish from other solid nonadenocarcinoma tumors. The diagnosis depends on the demonstration of acinar differentiation, obtained with antibodies recognizing various pancreatic enzymes that, although specific, show different sensitivity. The C-terminal portion of the BCL10 protein shows homology with carboxyl ester hydrolase (CEH), an enzyme produced by pancreatic acinar cells. We investigated the usefulness of a C-terminal BCL10 monoclonal antibody in the diagnosis of ACCs. We examined normal pancreases and different pancreatic tumors including ACCs, mixed acinar–endocrine carcinomas, ductal adenocarcinomas, mucinous, serous, solid pseudopapillary, and endocrine neoplasms. In addition, various normal tissues and cases of pancreatic metaplasia of the gastroesophageal mucosa, cases of ectopic pancreas, gastrointestinal endocrine tumors, salivary and breast acinic cell carcinomas, gastric adenocarcinomas with and without acinar differentiation, and hepatocellular carcinomas were studied. BCL10 immunoreactivity paralleled that of CEH and was restricted to acinar cells of normal and ectopic pancreas, of pancreatic metaplasia, and of ACCs. The anti-BCL10 antibody was more sensitive in detecting ACCs and pancreatic metaplasia than antibodies directed against other pancreatic enzymes. We suggest using BCL10 antibody for diagnosing pancreatic tumors and whenever an acinar differentiation is suspected in gastrointestinal neoplastic and metaplastic lesions.

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Acknowledgments

This study was supported by a grant from the University of Insubria, Varese. Silvia Marchet is the recipient of a fellowship from the “Varese per l’Oncologia” Association. The authors thank Anna and baby Riccardo La Rosa for providing samples of human milk and Dr. Luigi Terracciano (Institute of Pathology, University Hospital of Basel, Switzerland) for preparing the microarray slides.

Conflict of interest statement

We declare that we have no conflict of interest.

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Authors and Affiliations

  1. Department of Pathology, Ospedale di Circolo, Viale Borri 57, 21100, Varese, Italy

    Stefano La Rosa, Giovanna Finzi & Anna Maria Chiaravalli

  2. Anatomic Pathology Unit, Department of Human Morphology, University of Insubria, Varese, Italy

    Francesca Franzi, Silvia Marchet, Fausto Sessa & Carlo Capella

  3. Department of Biomedical, Experimental and Clinical Sciences, University of Insubria, Varese, Italy

    Moira Clerici & Davide Vigetti

  4. Department of Pathology, Fondazione IRCCS Multimedica, Milan, Italy

    Fausto Sessa

Authors
  1. Stefano La Rosa
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  2. Francesca Franzi
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  3. Silvia Marchet
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  4. Giovanna Finzi
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  5. Moira Clerici
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  6. Davide Vigetti
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  7. Anna Maria Chiaravalli
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  8. Fausto Sessa
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  9. Carlo Capella
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Correspondence to Stefano La Rosa.

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La Rosa, S., Franzi, F., Marchet, S. et al. The monoclonal anti-BCL10 antibody (clone 331.1) is a sensitive and specific marker of pancreatic acinar cell carcinoma and pancreatic metaplasia. Virchows Arch 454, 133–142 (2009). https://doi.org/10.1007/s00428-008-0710-x

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  • DOI: https://doi.org/10.1007/s00428-008-0710-x

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