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Comparative proteomic profiles of Pinus monticola needles during early compatible and incompatible interactions with Cronartium ribicola

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Abstract

The proteomic profiles of primary needles from Cr2-resistant and cr2-susceptible Pinus monticola seedlings were analysed post Cronartium ribicola inoculation by 2-DE. One hundred-and-five protein spots exhibiting significant differential expression were identified using LC–MS/MS. Functional classification showed that the most numerous proteins are involved in defence signalling, oxidative burst, metabolic pathways, and other physiological processes. Our results revealed that differential expression of proteins in response to C. ribicola inoculation was genotype- and infection-stage dependent. Responsive proteins in resistant seedlings with incompatible white pine blister rust (WPBR) interaction included such well-characterized proteins as heat shock proteins (HSPs), reactive oxygen species (ROS) scavenging enzymes, and intermediate factors functioning in the signal transduction pathways triggered by well-known plant R genes, as well as new candidates in plant defence like sugar epimerase, GTP-binding proteins, and chloroplastic ribonucleoproteins. Fewer proteins were regulated in susceptible seedlings; most of them were in common with resistant seedlings and related to photosynthesis among others. Quantitative RT-PCR analysis confirmed HSP- and ROS-related genes played an important role in host defence in response to C. ribicola infection. To the best of our knowledge, this is the first comparative proteomics study on WPBR interactions at the early stages of host defence, which provides a reference proteomic profile for other five-needle pines as well as resistance candidates for further understanding of host resistance in the WPBR pathosystem.

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Abbreviations

CAN:

Acetonitrile

2-DE:

Two-dimensional electrophoresis

H2O2 :

Hydrogen peroxide

Hpi:

Hours post inoculation

HSP:

Heat shock protein

HR:

Hypersensitive response

LC-MS/MS:

Liquid chromatography-tandem mass spectrometry

LRR:

Leucine-rich repeat

PRX:

Peroxiredoxin

RT-qPCR:

Quantitative reverse transcription-polymerase chain reaction

RLK:

Receptor-like kinase

ROS:

Reactive oxygen species

RuBisCO:

Ribulose-1,5-bisphosphate carboxylase oxygenase

SOD:

Superoxide dismutase

WWP:

Western white pine

WPBR:

White pine blister rust

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Acknowledgments

This research was supported in part by the Canadian Forest Service and the CFS-Genomics R&D Initiative fund awarded to JJL. We thank T. Holmes for the microscopy work, and Dr. E. Becker and H. Williams for their critical review of the manuscript.

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  1. Natural Resources Canada, Canadian Forest Service, Pacific Forestry Centre, 506 West Burnside Road, Victoria, BC, V8Z 1M5, Canada

    Arezoo Zamany, Jun-Jun Liu & Abul K. M. Ekramoddoullah

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  1. Arezoo Zamany
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  2. Jun-Jun Liu
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Correspondence to Jun-Jun Liu.

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Zamany, A., Liu, JJ. & Ekramoddoullah, A.K.M. Comparative proteomic profiles of Pinus monticola needles during early compatible and incompatible interactions with Cronartium ribicola . Planta 236, 1725–1746 (2012). https://doi.org/10.1007/s00425-012-1715-x

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