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Identification and fine mapping of a thermo-sensitive chlorophyll deficient mutant in rice (Oryza sativa L.)

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Abstract

A thermo-sensitive chlorophyll deficient mutant was isolated from more than 15,000 transgenic rice lines. The mutant displayed normal phenotype at 23°C or lower temperature (permissive temperature). However, when grown at 26°C or higher (nonpermissive temperature) the plant exhibited an abnormal phenotype characterized by yellow green leaves. Genetic analysis revealed that a single nuclear-encoded recessive gene is responsible for the mutation, which is tentatively designed as cde1(t) (chlorophyll deficient 1, temporally). PCR analysis and hygromycin resistance assay indicated the mutation was not caused by T-DNA insertion. To isolate the cde1(t) gene, a map-based cloning strategy was employed and 15 new markers (five SSR and ten InDels markers) were developed. A high-resolution physical map of the chromosomal region around the cde1(t) gene was made using F2 and F3 population consisting of 1,858 mutant individuals. Finally, the cde1(t) gene was mapped in 7.5 kb region between marker ID10 and marker ID11 on chromosome 2. Sequence analysis revealed only one candidate gene, OsGluRS, in the 7.5 kb region. Cloning and sequencing of the target region from the cde1(t) mutant showed that a missense mutation occurred in the mutant. So the OsGluRS gene (TIGR locus Os02 g02860) which encode glutamyl-tRNA synthetase was identified as the Cde1(t) gene.

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Abbreviations

cde1(t) :

Chlorophyll deficient 1, temporally

Chl:

Chlorophyll

OsGluRS :

Oryza glutamyl-tRNA synthetase

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Acknowledgments

We thank Dr Qifa Zhang (Huazhong Agricultural University, China) for providing us with the pSMR-J18R plasmid. This project was supported by the grants from the National Basic Research Priorities (973) Programmes of China (G19990116-1 and 2005CB120801) and National Natural Science Foundation of China (30623006). We are grateful to Mrs Honglan Yan (China National Rice Research Institute, China) for taking pictures for the article.

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Authors and Affiliations

  1. College of Life Sciences, Zhejiang University, 310029, Hangzhou, ZJ, China

    Wenzhen Liu

  2. State Key Laboratory of Rice Biology, China National Rice Research Institute, 359 Tiyuchang Road, 310006, Hangzhou, ZJ, China

    Wenzhen Liu, Yaping Fu, Guocheng Hu, Huamin Si, Li Zhu, Chao Wu & Zongxiu Sun

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  1. Wenzhen Liu
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  2. Yaping Fu
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Correspondence to Zongxiu Sun.

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Liu, W., Fu, Y., Hu, G. et al. Identification and fine mapping of a thermo-sensitive chlorophyll deficient mutant in rice (Oryza sativa L.). Planta 226, 785–795 (2007). https://doi.org/10.1007/s00425-007-0525-z

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  • DOI: https://doi.org/10.1007/s00425-007-0525-z

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