Abstract
Two class III peroxidases from Arabidopsis, AtPrx33 and Atprx34, have been studied in this paper. Their encoding genes are mainly expressed in roots; AtPrx33 transcripts were also found in leaves and stems. Light activates the expression of both genes in seedlings. Transformed seedlings producing AtPrx33-GFP or AtPrx34-GFP fusion proteins under the control of the CaMV 35S promoter exhibit fluorescence in the cell walls of roots, showing that the two peroxidases are localized in the apoplast, which is in line with their affinity for the Ca2+-pectate structure. The role they can play in cell wall was investigated using (1) insertion mutants that have suppressed or reduced expression of AtPrx33 or AtPrx34 genes, respectively, (2) a double mutant with no AtPrx33 and a reduced level of Atprx34 transcripts, (3) a mutant overexpressing AtPrx34 under the control of the CaMV 35S promoter. The major phenotypic consequences of these genetic manipulations were observed on the variation of the length of seedling roots. Seedlings lacking AtPrx33 transcripts have shorter roots than the wild-type controls and roots are still shorter in the double mutant. Seedlings overexpressing AtPrx34 exhibit significantly longer roots. These modifications of root length are accompanied by corresponding changes of cell length. The results suggest that AtPrx33 and Atprx34, two highly homologous Arabidopsis peroxidases, are involved in the reactions that promote cell elongation and that this occurs most likely within cell walls.
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Abbreviations
- CaMV 35S:
-
Cauliflower mosaic virus 35S promoter
- CTPP:
-
C-terminal propeptide
- DAPI:
-
4’,6-diamidino-2-phenylindole
- GFP:
-
green fluorescent protein
- ROS:
-
reactive oxygen species
- RT-PCR:
-
reverse-transcriptase polymerase chain reaction
- WAK:
-
wall associated kinase
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Acknowledgements
We thank Prof. Susan Gasser (Friedrich Miescher Institute, FMI, Basel, Switzerland) for the use of the Zeiss Axioplan II microscope, Dr. Thierry Laroche (FMI) for his scientific comments and technical support, and Prof. Christian Fankhauser (University of Lausanne, Switzerland) for the gift of the binary vectors pCHF3 and pCHF5. This work was supported by the Swiss National Science Foundation (grant 31-068003.02).
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Passardi, F., Tognolli, M., De Meyer, M. et al. Two cell wall associated peroxidases from Arabidopsis influence root elongation. Planta 223, 965–974 (2006). https://doi.org/10.1007/s00425-005-0153-4
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DOI: https://doi.org/10.1007/s00425-005-0153-4