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Chilling stress suppresses chloroplast development and nuclear gene expression in leaves of mung bean seedlings

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Abstract

Etiolated leaves of 28°C-dark-grown mung bean (Vigna radiata L. cv. 2937) seedlings fail to turn green after being shifted to a light and cold environment. At the visible phenotypic level, incapability of leaf greening is the only failure event for the de-etiolation of mung bean seedlings at low temperature. Ultrastructural studies revealed that chloroplast development was completely suppressed by chilling treatment. A cDNA library originating from 28°C-light-grown seedling leaves was constructed for screening cold-suppressed (cos) genes. Thirteen full-length cDNA clones were obtained, with 12 clones encoding chloroplast proteins, which, according to their known physiological functions, were important for chloroplast development and photosynthesis. Another cos cDNA encodes CYP90A2, which is a cytochrome P450 protein involved in the biosynthesis of brassinosteroid hormones. All cos genes are light-regulated at normal temperature. The influence of chilling stress on cos expression was examined in 10°C-light- and 10°C-dark-grown etiolated seedlings, and in 10°C-light-grown green plants. The data show that cos expression in these three treatments is severely suppressed. This suppression is controlled at the transcriptional level, as demonstrated by nuclear runoff experiments, and is reversible because cos mRNAs accumulate again after the cold-treated plants have been transferred to 28°C.

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Abbreviations

β-CA:

β-Carbonic anhydrase

cos :

Cold suppressed

CTIC:

Cool-temperature-induced-chlorosis

LHC II:

Light-harvesting complex II

POR:

Protochlorophyllide oxidoreductase

PSI:

Photosystem I

PSII:

Photosystem II

RCA:

Rubisco activase

SSU:

Rubisco small subunit

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Acknowledgements

The authors are grateful to Dr. David R. Nelson (University of Tennessee, Memphis, USA) for the assignment of the COS10 sequence to CYP90A2. This investigation was supported by grants from the National Science Council, ROC (Taiwan) (NSC86-2311-B002-009, NSC87-2311-B002-005-B01 and NSC88-2311-B002-018).

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Authors and Affiliations

  1. Institute of Plant Biology, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd, Taipei, 106, Taiwan (ROC)

    Ming-Tzong Yang, Shu-Ling Chen, Chu-Yung Lin & Yih-Ming Chen

  2. Department of Biotechnology, Ming Chuan University, Taoyuan, 333, Taiwan (ROC)

    Shu-Ling Chen

  3. Agriculture and Food Agency, Council of Agriculture, Taiwan (ROC)

    Ming-Tzong Yang

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  1. Ming-Tzong Yang
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  2. Shu-Ling Chen
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Correspondence to Yih-Ming Chen.

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Yang, MT., Chen, SL., Lin, CY. et al. Chilling stress suppresses chloroplast development and nuclear gene expression in leaves of mung bean seedlings. Planta 221, 374–385 (2005). https://doi.org/10.1007/s00425-004-1451-y

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