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Association of PTPN22 gene (rs2488457) polymorphism with ulcerative colitis and high levels of PTPN22 mRNA in ulcerative colitis

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International Journal of Colorectal Disease Aims and scope Submit manuscript

Abstract

Purpose

Our aims were to evaluate protein tyrosine phosphatase nonreceptor type 22 (PTPN22) gene polymorphisms in ulcerative colitis (UC) and explore PTPN22 mRNA levels in colonic biopsies of UC patients in central China.

Methods

A total of 165 Chinese UC patients and 300 healthy controls were enrolled in this study. PTPN22 −1123G/C, +1858C/T, and +788G/A polymorphisms were genotyped by PCR-restriction fragment length polymorphism method. PTPN22 mRNA expressions in colonic biopsies and serum C-reactive protein (CRP) levels were determined by quantitative PCR and immunonephelometry, respectively.

Results

The frequency of C carrier was higher in UC patients than in healthy controls (66.7 vs. 53.3 %, P = 0.005, odds ratios = 1.75, 95 % CI 1.18–2.60) and associated with extensive colitis (P = 0.029). PTPN22 mRNA levels were elevated in UC patients than in healthy controls (P < 0.001). Among UC patients, PTPN22 mRNA expression levels were higher in biopsies of inflamed colonic tissue compared with noninflamed tissue (P < 0.001) and were correlated with CRP levels (r = 0.578, P < 0.001). PTPN22 mRNA expression levels were elevated in extensive colitis compared to proctitis (P = 0.008) and to left-sided colitis (P = 0.029) and were higher in moderate and severe disease than in mild disease (P = 0.005).

Conclusions

Our study showed the potential association between PTPN22 −1123G/C polymorphism and UC in central China. PTPN22 mRNA levels were highly expressed in UC, especially in active disease, and were correlated with CRP levels, disease location, and disease severity in UC patients.

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Acknowledgments

This project was supported by grants from the Bureau of Public Health of Wuhan (WX12C34).

Conflict of interest

The authors declare that they have no competing interests.

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Authors and Affiliations

  1. Department of Gastroenterology and Central Laboratory, The Central Hospital of Wuhan, Sheng Li Street 26, Wuhan, 430014, Hubei Province, People’s Republic of China

    Zhitao Chen, Heng Zhang, Ping Wang, Ting Jiang, Min Song & Jie Wu

  2. Department of Gastroenterology, Wuhan University Zhongnan Hospital, Wuhan, China

    Bing Xia

  3. Clinical Research Center for Intestinal and Colorectal Diseases of Hubei Province, Wuhan, China

    Bing Xia

  4. Key Laboratory of Allergy and Immune-related Diseases, Wuhan, 430071, People’s Republic of China

    Bing Xia

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  1. Zhitao Chen
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  2. Heng Zhang
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  3. Bing Xia
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  4. Ping Wang
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  5. Ting Jiang
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  6. Min Song
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  7. Jie Wu
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Corresponding author

Correspondence to Jie Wu.

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Chen, Z., Zhang, H., Xia, B. et al. Association of PTPN22 gene (rs2488457) polymorphism with ulcerative colitis and high levels of PTPN22 mRNA in ulcerative colitis. Int J Colorectal Dis 28, 1351–1358 (2013). https://doi.org/10.1007/s00384-013-1671-3

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  • DOI: https://doi.org/10.1007/s00384-013-1671-3

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