Abstract
Purpose
Idiopathic asthenozoospermia is considered as one of the causes of male infertility and characterized by reduced sperm motility. For a better determination of pathogenic mechanism of asthenozoospermia, the exploration of differentially expressed proteins in normal sperm motility and idiopathic asthenozoospermia was conducted in our study.
Methods
Sperm proteins were extracted and isolated by two-dimensional electrophoresis. All significantly changed protein spots were picked up from 2D gels and identified by tandem mass spectrometry. Sixteen of the thirty-three total differentially expressed protein spots were successfully identified by matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry.
Results
Sixteen proteins identified belonged to 15 unique protein groups. GRP78, lactoferrin, SPANXB, PGK2, flagellin, DJ-1, XPA binding protein 2, CAB2, GPX4, and GAPDH were the first to be identified as differentially expressed proteins in idiopathic asthenospermia patients. Meanwhile, the analysis of quantitative RT-PCR was carried out to compare the protein levels, and the results indicated that the expression levels of the gene and protein were not entirely consistent.
Conclusions
These experimental results expand the scope of the protein database, generating targets for further investigation of the pathogenic mechanism of idiopathic asthenozoospermia.
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Acknowledgments
The work was supported by National Natural Science Foundation (No. 81260123) and Nature Science Foundation of the Guangxi Zhuang Autonomous Region to Shulin Shen (No. Gui ke zi 0991271).
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The authors declare no conflict of interest.
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Shulin Shen and Jinzi Wang contributed equally to this work.
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Shen, S., Wang, J., Liang, J. et al. Comparative proteomic study between human normal motility sperm and idiopathic asthenozoospermia. World J Urol 31, 1395–1401 (2013). https://doi.org/10.1007/s00345-013-1023-5
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DOI: https://doi.org/10.1007/s00345-013-1023-5