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Adventitious Shoot Induction from Internode and Root Explants in a Semiparasitic Herb Monochasma savatieri Franch ex Maxim

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Abstract

A highly efficient protocol for the induction of adventitious shoots from young internode and root explants of a semiparasitic medicinal herb Monochasma savatieri Franch ex Maxim was developed. MS basal medium supplemented with 5 µM thidiazuron (TDZ) induced 32 adventitious shoots/explant, which was double the number obtained using the same concentration of 6-benzyladenine (BA). Hyperhydric shoots were observed when 10 µM of any cytokinin was added to MS media. Use of any cytokinin at 2.5 µM produced an average of 14–21 adventitious shoots/root explant. Shoots formed roots in vitro more effectively than α-naphthaleneacetic acid when indole-3-butyric acid and indole-3-acetic acid were used at 1.0 µM. Two-month-old rooted plantlets were transplanted to vermiculite and 70% survived after 4 months.

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Authors Contribution

Conceived and designed the experiments: Xinhua Zhang, Jaime A. Teixeira da Silva, Guohua Ma. Performed the experiments: Yueya Zhang, Yulu Chen. Wrote the paper, analyzed the data and edited all versions: Yueya Zhang, Yulu Chen, Xinhua Zhang, Jaime A. Teixeira da Silva, Guohua Ma.

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    Authors and Affiliations

    1. Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, the Chinese Academy of Sciences, Guangzhou, 510650, China

      Yueya Zhang, Yulu Chen, Xinhua Zhang & Guohua Ma

    2. University of the Chinese Academy of Sciences, Beijing, China

      Yueya Zhang & Yulu Chen

    3. P. O. Box 7, Miki-cho post office, Ikenobe 3011-2, Miki-cho, Kagawa-ken, 761-0799, Japan

      Jaime A. Teixeira da Silva

    Authors
    1. Yueya Zhang
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    2. Yulu Chen
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    3. Xinhua Zhang
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    4. Jaime A. Teixeira da Silva
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    5. Guohua Ma
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    Correspondence to Xinhua Zhang, Jaime A. Teixeira da Silva or Guohua Ma.

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    The authors declare no conflicts of interest.

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    Yueya Zhang and Yulu Chen have contributed equally.

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    Zhang, Y., Chen, Y., Zhang, X. et al. Adventitious Shoot Induction from Internode and Root Explants in a Semiparasitic Herb Monochasma savatieri Franch ex Maxim. J Plant Growth Regul 36, 799–804 (2017). https://doi.org/10.1007/s00344-017-9681-y

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    • DOI: https://doi.org/10.1007/s00344-017-9681-y

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