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A WRKY transcription factor, PcWRKY33, from Polygonum cuspidatum reduces salt tolerance in transgenic Arabidopsis thaliana

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PcWRKY33 is a transcription factor which can reduce salt tolerance by decreasing the expression of stress-related genes and increasing the cellular levels of reactive oxygen species (ROS).

Abstract

WRKY transcription factors play important roles in the regulation of biotic and abiotic stresses. Here, we report a group I WRKY gene from Polygonum cuspidatum, PcWRKY33, that encodes a nucleoprotein, which specifically binds to the W-box in the promoter of target genes to regulate their expression. The results from qPCR and promoter analysis show that expression of PcWRKY33 can be induced by various abiotic stresses, including NaCl and plant hormones. Overexpression of PcWRKY33 in Arabidopsis thaliana reduced tolerance to salt stress. More specifically, several physiological parameters (such as root length, seed germination rate, seedling survival rate, and chlorophyll concentration) of the transgenic lines were significantly lower than those of the wild type under salt stress. In addition, following exposure to salt stress, transgenic plants showed decreased expression of stress-related genes, a weakened ability to maintain Na+/K+ homeostasis, decreased activities of reactive oxygen species- (ROS-) scavenging enzymes, and increased accumulation of ROS. Taken together, these results suggest that PcWRKY33 negatively regulates the salt tolerance in at least two ways: by down-regulating the induction of stress-related genes and by increasing the level of cellular ROS. In sum, our results indicate that PcWRKY33 is a group I WRKY transcription factor involved in abiotic stress regulation.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (Grant No.61672489; 61379081).

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Authors and Affiliations

  1. College of Life Sciences, University of Chinese Academy of Sciences, Beijing, 101408, China

    Wenqi Bao, Xiaowei Wang, Mo Chen, Tuanyao Chai & Hong Wang

  2. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China

    Tuanyao Chai

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  1. Wenqi Bao
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  2. Xiaowei Wang
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  3. Mo Chen
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  4. Tuanyao Chai
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  5. Hong Wang
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Correspondence to Tuanyao Chai or Hong Wang.

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Communicated by Kang Chong.

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Bao, W., Wang, X., Chen, M. et al. A WRKY transcription factor, PcWRKY33, from Polygonum cuspidatum reduces salt tolerance in transgenic Arabidopsis thaliana. Plant Cell Rep 37, 1033–1048 (2018). https://doi.org/10.1007/s00299-018-2289-2

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