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The physiology of ex vitro pineapple (Ananas comosus L. Merr. var MD-2) as CAM or C3 is regulated by the environmental conditions: proteomic and transcriptomic profiles

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Abstract

Key message

Proteomic and transcriptomic profiles of key enzymes were monitored in pineapple plants propagated under C3 and CAM-inducing metabolisms to obtain insight into the CAM-facultative metabolism and the relationship of CAM plants with oxidative stress.

Abstract

Pineapple is one of the most important tropical crops worldwide. The use of temporary immersion bioreactors for the first stages of pineapple propagation enables precise control of plant growth, increases the rate of plant multiplication, decreases space, energy and labor requirements for pineapple plants in commercial micropropagation. Once the plantlets are ready to be taken from the reactors, they are carefully acclimatized to natural environmental conditions, and a facultative C3/CAM metabolism in the first 2 months of growth is the characteristic of pineapple plants, depending on environmental conditions. We subjected two sets of micropropagated pineapple plants to C3 and CAM-inducing environmental conditions, determined by light intensity/relative humidity (respectively 40 μmol m−2 s−1/85 % and 260 μmol m−2 s−1/50 %). Leaves of pineapple plants grown under CAM-inducing conditions showed higher leaf thickness and more developed cuticles and hypodermic tissue. Proteomic profiles of several proteins, isoenzyme patterns and transcriptomic profiles were also measured. Five major spots were isolated and identified, two of them for the first time in Ananas comosus (OEE 1; OEE 2) and the other three corresponding to small fragments of the large subunit of Rubisco (LSU). PEPC and PEPCK were also detected by immunobloting of 2DE at the end of both ex vitro treatments (C3/CAM) during the dark period. Isoenzymes of SOD and CAT were identified by electrophoresis and the transcript levels of OEE 1 and CAT were associated with CAM metabolism in pineapple plants.

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Abbreviations

APX:

Ascorbate peroxidase

CAT:

Catalase

GR:

Glutathione reductase

LSU:

Rubisco large subunit

OEE:

Oxygen Evolving Enhancer

PEPC:

Phosphoenolpyruvate carboxylase

PEPCK:

Phosphoenolpyruvate carboxykinase

Rubisco:

Ribulose-1,5-bisphosphate carboxylase oxygenase

SOD:

Superoxide dismutase

SSU:

Rubisco small subunit

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Acknowledgments

Acknowledgments to Fundação para a Ciência e Tecnologia (FCT) for the financial support to CBAA (PEst-OE/AGR/UI0240/2011) and the PhD grant SFRH/BD/43181/2008 to CA.

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Authors and Affiliations

  1. Universidade de Lisboa, DRAT, CBAA, ISA, Tapada da Ajuda, 1349-017, Lisbon, Portugal

    C. Aragón, P. Pascual, L. Carvalho & S. Amancio

  2. Laboratory for Plant Cell and Tissue Culture, Centro de Bioplantas, Universidad de Ciego de Ávila, Ciego de Ávila, Cuba

    C. Aragón, J. González & M. Escalona

Authors
  1. C. Aragón
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  2. P. Pascual
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  3. J. González
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  4. M. Escalona
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  5. L. Carvalho
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  6. S. Amancio
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Correspondence to S. Amancio.

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Communicated by P. Puigdomenech.

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Aragón, C., Pascual, P., González, J. et al. The physiology of ex vitro pineapple (Ananas comosus L. Merr. var MD-2) as CAM or C3 is regulated by the environmental conditions: proteomic and transcriptomic profiles. Plant Cell Rep 32, 1807–1818 (2013). https://doi.org/10.1007/s00299-013-1493-3

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  • DOI: https://doi.org/10.1007/s00299-013-1493-3

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