Skip to main content
SpringerLink
Log in

Expressed sequence tags from persimmon at different developmental stages

  • Genetics and Genomics
  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

Persimmon (Diospyros kaki Thunb.) is an important fruit in Asian countries, where it is eaten as a fresh fruit and is also used for many other purposes. To understand the molecular mechanism of fruit development and ripening in persimmon, we generated a total of 9,952 expressed sequence tags (ESTs) from randomly selected clones of two different cDNA libraries. One cDNA library was derived from fruit of “Saijo” persimmon at an early stage of development, and the other from ripening fruit. These ESTs were clustered into 6,700 non-redundant sequences. Of the 6,700 non-redundant sequences evaluated, the deduced amino acid sequences of 4,356 (65%) showed significant homology to known proteins, and 2,344 (35%) showed no significant similarity to any known proteins in Arabidopsis databases. We report comparison of genes identified in the two cDNA libraries and describe some putative genes involved in proanthocyanidin and carotenoid synthesis. This study provides the first global overview of a set of genes that are expressed during fruit development and ripening in persimmon.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403–410

    PubMed  CAS  Google Scholar 

  • Berardini TZ, Mundodi S, Reiser L, Huala E, Garcia-Hernandez M, Zhang P, Mueller LA, Yoon J, Doyle A, Lander G, Moseyko N, Yoo D, Xu I, Zoeckler B, Montoya M, Miller N, Weems D, Rhee SY (2004) Functional annotation of the Arabidopsis genome using controlled vocabularies. Plant Physiol 135:745–755. doi:10.1104/pp.104.040071

    Article  PubMed  CAS  Google Scholar 

  • Camardella L, Carratore V, Ciardiello MA, Servillo L, Balestrieri C, Giovane A (2000) Kiwi protein inhibitor of pectin methylesterase. Amino-acid sequence and structural importance of two disulfide bridges. Eur J Biochem 267:4561–4565. doi:10.1046/j.1432-1327.2000.01510.x

    Article  PubMed  CAS  Google Scholar 

  • Chang JJ, Chen TH, Chan P, Chen YJ, Hsu FL, Lo MY, Lin JY (2001) The in vitro inhibitory effect of tannin derivatives on 3-hydroxy-3-methylglutaryl- coenzyme A reductase on Vero cells. Pharmacology 62:224–228. doi:10.1159/000056099

    Article  PubMed  CAS  Google Scholar 

  • Chang S, Puryear J, Cairney J (1993) A simple and efficient method for isolating RNA from pine trees. Plant Mol Biol Rep 11:113–116. doi:10.1007/BF02670468

    Article  CAS  Google Scholar 

  • Chen MH, Sheng J, Hind G, Handa AK, Citovsky V (2000) Interaction between the tobacco mosaic virus movement protein and host cell pectin methylesterase is required for viral cell-to-cell movement. EMBO J 19:913–920. doi:10.1093/emboj/19.5.913

    Article  PubMed  CAS  Google Scholar 

  • Da Silva FG, Iandolino A, Al-Kayal F, Bohlmann MC, Cushman MA, Lim H, Ergul A, Figueroa R, Kabuloglu EK, Osborne C, Rowe J, Tattersall E, Leslie A, Xu J, Baek J, Cramer GR, Cushman JC, Cook DR (2005) Characterizing the grape transcriptome. Analysis of expressed sequence tags from multiple Vitis species and development of a compendium of gene expression during berry development. Plant Physiol 139:574–597. doi:10.1104/pp.105.065748

    Article  PubMed  CAS  Google Scholar 

  • Devitt LC, Sawbridge T, Holton TA, Mitchelson K, Dietzgen RG (2006) Discovery of genes associated with fruit ripening in Carica papaya using expressed sequence tags. Plant Sci 170:356–363. doi:10.1016/j.plantsci.2005.09.003

    Article  CAS  Google Scholar 

  • Ewing B, Hillier L, Wendl MC, Green P (1998) Base-calling of automated sequencer traces using phred. I Accuracy assessment. Genome Res 8:175–185. doi:10.1101/gr.8.3.175

    PubMed  CAS  Google Scholar 

  • Fei Z, Tang X, Alba RM, White JA, Ronning CM, Martin GB, Tanksley SD, Giovannoni JJ (2004) Comprehensive EST analysis of tomato and comparative genomics of fruit ripening. Plant J 40:47–59. doi:10.1111/j.1365-313X.2004.02188.x

    Article  PubMed  Google Scholar 

  • Fujiki H (1999) Two stages of cancer prevention with green tea. J Cancer Res Clin Oncol 125:589–597. doi:10.1007/s004320050321

    Article  PubMed  CAS  Google Scholar 

  • Fujiki H (2005) Green tea: health benefits as cancer preventive for humans. Chem Rec 5:119–132

    Article  PubMed  CAS  Google Scholar 

  • Giovane A, Balestrieri C, Quagliuolo L, Castaldo D, Servillo L (1995) A glycoprotein inhibitor of pectin methylesterase in kiwi fruit. Purification by affinity chromatography and evidence of a ripening-related precursor. Eur J Biochem 233:926–929. doi:10.1111/j.1432-1033.1995.926_3.x

    Article  PubMed  CAS  Google Scholar 

  • Gray J, Picton S, Shabbeer J, Schuch W, Grierson D (1992) Molecular biology of fruit ripening and its manipulation with antisense genes. Plant Mol Biol 19:69–87. doi:10.1007/BF00015607

    Article  PubMed  CAS  Google Scholar 

  • Grimplet J, Romieu C, Audergon JM, Marty I, Albagnac G, Lambert P, Bouchet JP, Terrier N (2005) Transcriptomic study of apricot fruit (Prunus armeniaca) ripening among 13,006 expressed sequence tags. Physiol Plant 125:281–292. doi:10.1111/j.1399-3054.2005.00563.x

    Article  Google Scholar 

  • Hirano K, Yonemori K, Sugiura A (1995) Involvement of sugar metabolism in persimmon growth-inhibition by calyx lobe removal. J Am Soc Hortic Sci 120:75–77

    CAS  Google Scholar 

  • Ikegami A, Kitajima A, Yonemori K (2005a) Inhibition of flavonoid biosynthetic gene expression coincides with loss of astringency in pollination-constant, non-astringent (PCNA)-type persimmon fruit. J Hortic Sci Biotechnol 80:225–228

    CAS  Google Scholar 

  • Ikegami A, Yonemori K, Kitajima A, Sato A, Yamada M (2005b) Expression of genes involved in proanthocyanidin biosynthesis during fruit development in a Chinese pollination-constant, nonastringent (PCNA) persimmon, ‘Luo Tian Tian Shi’. J Am Soc Hortic Sci 130:830–835

    CAS  Google Scholar 

  • Ikegami A, Eguchi S, Kitajima A, Inoue K, Yonemori K (2007) Identificaiton of genes involved in proanthocyanidin biosynthesis of persimmon (Diospyros kaki) fruit. Plant Sci 172:1037–1047. doi:10.1016/j.plantsci.2007.02.010

    Article  CAS  Google Scholar 

  • Itamura H, Kitamura T, Taira S, Harada H, Ito N, Takahashi Y, Fukushima T (1991) Relationship between fruit softening, ethylene production and respiration in Japanese persimmon ‘Hiratanenashi’. J Japan Soc Hortic Sci 60:695–701. (Japanese with English summary)

    Article  CAS  Google Scholar 

  • Itamura H, Ohno Y, Yamamura H (1997) Characteristics of fruit softening in Japanese persimmon. Acta Hortic 436:179–188

    CAS  Google Scholar 

  • Kanayama Y (2006) Horticultural statistics of Japan. In: The Japanese society of horticultural science (ed) Horticulture in Japan 2006. Nakanishi Printing Co. Ltd, Kyoto, pp 15–20

  • Kitagawa H, Glucina PG (1984) Cause and removal astringency. In: Ruscoe QW (eds) Persimmon culture in New Zealand. Science Information Publishing Centre, Wellington, pp 55–61

    Google Scholar 

  • Levi A, Davis A, Hernandez A, Wechter P, Thimmapuram J, Trebitsh T, Tadmor Y, Katzir N, Portnoy V, King S (2006) Genes expressed during the development and ripening of watermelon fruit. Plant Cell Rep 25:1233–1245. doi:10.1007/s00299-006-0163-0

    Article  PubMed  CAS  Google Scholar 

  • Matsuo T, Ito S (1978) The chemical structure of kaki-tannin from immature fruit of the persimmon (Diospyros kaki L.). Agric Biol Chem 42:1637–1643

    CAS  Google Scholar 

  • Matsuo T, Kinoshita M, Itoo S, Shimoi K, Okada Y, Tomita I (1991) Antimutagenic effect of Kaki-shibu and its tannin degraded products on UV-induced mutagenesis in Escherichia coli. J Japan Soc Hortic Sci 60:429–435, (Japanese with English summary)

    CAS  Google Scholar 

  • Mekhfi H, ElHaouari M, Bnouham M, Aziz M, Ziyyat A, Legssyer A (2006) A Effects of extracts and tannins from Arbutus unedo leaves on rat platelet aggregation. Phytother Res 20:135–139. doi:10.1002/ptr.1822

    Article  PubMed  CAS  Google Scholar 

  • Moser C, Segala C, Fontana P, Salakhudtinov I, Gatto P, Pindo M, Zyprian E, Toepfer R, Grando MS, Velasco R (2005) Comparative analysis of expressed sequence tags from different organs of Vitis vinifera L. Funct Integr Genomics 5:208–217. doi:10.1007/s10142-005-0143-4

    Article  PubMed  CAS  Google Scholar 

  • Moyle R, Fairbairn DJ, Ripi J, Crowe M, Botella JR (2005) Developing pineapple fruit has a small transcriptome dominated by metallothionein. J Exp Bot 56:101–112. doi:10.1093/jxb/eri015

    PubMed  CAS  Google Scholar 

  • Newcomb RD, Crowhurst RN, Gleave AP, Rikkerink EHA, Allan AC, Beuning LL, Bowen JH, Gera E, Jamieson KR, Janssen BJ, Laing WA, McArtney S, Nain B, Ross GS, Snowden KC, Souleyre EJF, Walton EF, Yauk Y-K (2006) Analyses of expressed sequence tags from apple. Plant Physiol 141:147–166. doi:10.1104/pp.105.076208

    Article  PubMed  Google Scholar 

  • Niikawa T, Suzuki T, Ozeki T, Kato M, Ikoma Y (2007) Characteristics of carotenoid accumulation during maturation of the Japanese persimmon ‘Fuyu’. Hort Res (Japan) 6:251–256, (Japanese with English summary)

    Article  CAS  Google Scholar 

  • Ogata S (1976) Waruyoi to futsukayoi no hatsugenkijo kara mita kakinomi no alcohol-meitei e no kouka. Jyo-kyo 71:488–495, (Japanese)

    CAS  Google Scholar 

  • Okuma Y, Ishikawa H, Ito Y, Hayashi Y, Endo A, Watanabe T (1995) Effect of extracts from Hovenia dulcis Thunb. on alcohol concentration in rats and men administered alcohol. J Jpn Soc Nutr Food Sci 48:167–172, (Japanese with English summary)

    CAS  Google Scholar 

  • Rhee SY, Beavis W, Berardini TZ, Chen G, Dixon D, Doyle A, Garcia-Hernandez M, Huala E, Lander G, Montoya M, Miller N, Mueller LA, Mundodi S, Reiser L, Tacklind J, Weems DC, Wu Y, Xu I, Yoo D, Yoon J, Zhang P (2003) The Arabidopsis information resource (TAIR): a model organism database providing a centralized, curated gateway to Arabidopsis biology, research materials and community. Nucleic Acids Res 31:224–228. doi:10.1093/nar/gkg076

    Article  PubMed  CAS  Google Scholar 

  • Sasakawa Z (1955) Clinical studies on the depressor effects of SHIBU against hypertension. J Jpn Soc Intern Med 43:858–866, (Japanese with English summary)

    Google Scholar 

  • Taira S (1996) Astringency in persimmon. In: Linskens HF, Jackson JF (eds) Fruit analysis. Modern methods of plant analysis. vol 18, Springer, Berlin, pp 97–110

    Google Scholar 

  • Terrier N, Ageorges A, Abbal P, Romieu C (2001) Generation of ESTs from grape berry at various developmental stages. J Plant Physiol 158:1575–1583. doi:10.1078/0176-1617-00566

    Article  CAS  Google Scholar 

  • Yang Y, Sulpice R, Himmelbach A, Meinhard M, Christmann A, Grill E (2006) Fibrillin expression is regulated by abscisic acid response regulators and is involved in abscisic acid-mediated photoprotection. Proc Natl Acad Sci USA 103:6061–6066. doi:10.1073/pnas.0501720103

    Article  PubMed  CAS  Google Scholar 

  • Yonemori K (1997) Persimmon industry and research activities in Japan. Acta Hortic 436:21–32

    Google Scholar 

  • Zheng GH, Sugiura A (1990) Changes in sugar composition in relation to invertase activity in the growth and ripening of persimmon (Diospyros kaki) fruit. J Japan Soc Hortic Sci 59:281–287, (Japanese with English summary)

    Article  Google Scholar 

Download references

Acknowledgments

This work was supported in part by Grant-in-Aid for Scientific Research (No 17380023) and Grant-in-Aid for Exploratory Research (No 19651084) from the Ministry of Education, Science and Culture, Japan.

Author information

Authors and Affiliations

  1. Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University, Matsue, 690-8504, Japan

    T. Nakagawa

  2. Faculty of Life and Environmental Sciences, Shimane University, Matsue, 690-8504, Japan

    A. Nakatsuka, S. Yasugahira, R. Nakamura, N. Sun & H. Itamura

  3. Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, 113-8657, Japan

    K. Yano

  4. Faculty of Agriculture, Tottori University, Tottori, 680-8533, Japan

    A. Itai

  5. Graduate School of Science, Nagoya University, Nagoya, 464-8601, Japan

    T. Suzuki

Authors
  1. T. Nakagawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Nakatsuka
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. K. Yano
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. S. Yasugahira
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. R. Nakamura
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. N. Sun
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. A. Itai
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. T. Suzuki
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. H. Itamura
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. Nakatsuka.

Additional information

Communicated by P. Puigdomenech.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nakagawa, T., Nakatsuka, A., Yano, K. et al. Expressed sequence tags from persimmon at different developmental stages. Plant Cell Rep 27, 931–938 (2008). https://doi.org/10.1007/s00299-008-0518-9

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00299-008-0518-9

Keywords

Search

Navigation