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Utility of the FLP-FRT recombination system for genetic manipulation of rice

  • Genetic Transformation and Hybridization
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Abstract

To develop an FLP-FRT recombination system- (derived from 2 μ plasmid of Saccharomyces cerevisiae) based marker gene removal application for rice, we introduced the gene for FLP recombinase, under the control of the maize ubiquitin-1 promoter, into the rice genome. FLP activity was monitored in callus and regenerated plants by an assay based on the deletion of the FRT-flanked DNA fragment, leading to the activation of the β-glucuronidase gene. FLP activity was detected both in the callus and leaves of some of the transgenic lines. Based on our comparison of the recombination efficiency of the FLP-FRT system expressed in the transgenic lines with that of the widely used Cre-lox system (derived from bacteriophage P1), we suggest that the FLP-FRT system is a useful tool for the genetic manipulation of rice.

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Abbreviations

GUS:

β-Glucuronidase

CaMV 35S:

Cauliflower Mosaic Virus 35S promoter

hpt :

Hygromycin phosphotransferase gene

nos3′:

Transcription termination sequence of nopaline synthase gene

npt :

Neomycin phosphotransferase gene

ubi-1 :

Maize ubiquitin-1 promoter

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Acknowledgements

This work was funded by the Arkansas Biosciences Institute and Arkansas Rice Research Board. We thank Drs. T. K. Hodges and David W. Ow for providing the plasmids.

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Authors and Affiliations

  1. Department of Crop, Soil and Environmental Sciences, University of Arkansas, Fayetteville, AR, 72701, USA

    Parthiban Radhakrishnan & Vibha Srivastava

  2. Department of Horticulture, University of Arkansas, Fayetteville, AR, 72701, USA

    Vibha Srivastava

Authors
  1. Parthiban Radhakrishnan
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  2. Vibha Srivastava
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Correspondence to Vibha Srivastava.

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Communicated by G.C. Phillips

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Radhakrishnan, P., Srivastava, V. Utility of the FLP-FRT recombination system for genetic manipulation of rice. Plant Cell Rep 23, 721–726 (2005). https://doi.org/10.1007/s00299-004-0876-x

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  • DOI: https://doi.org/10.1007/s00299-004-0876-x

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