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Apoptosis-related Fas and FasL gene polymorphisms’ associations with knee osteoarthritis

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Abstract

To investigate the associations between Fas and FasL gene polymorphisms and susceptibility to knee osteoarthritis. Genomic DNA was obtained from 146 patients with knee osteoarthritis and 102 healthy controls. Genotype distributions and allelic frequencies of four polymorphisms of Fas (-670 G>A rs1800682, -1377 G>A rs2234767) and FasL (IVS2nt-124 A>G rs5030772, -844 T>C rs763110) genes were compared between the groups. Thereafter, this association was investigated between patients and controls of the same sex. There were significant differences between patients with knee osteoarthritis and controls regarding the genotype distributions and allelic frequencies of Fas-1377 G>A polymorphism (P = 0.0001 and P = 0.005, respectively). The Fas-1377 GG genotype and G allele were significantly more frequent in patients with knee osteoarthritis than in controls. Genotype distributions and allelic frequencies of Fas-670 G>A, FasL-844 T>C, and FasL IVS2nt-124 A>G polymorphisms did not differ between the groups (P > 0.05). However, there were no significant differences between patients and controls of the same sex (P > 0.05). These findings suggest that the Fas-1377 G>A polymorphism in the Fas gene related with apoptosis may contribute to susceptibility to knee osteoarthritis in the Turkish population. There is a need for further studies to evaluate the role of apoptosis in large cohorts.

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Acknowledgments

This was not an industry-supported study. All authors have reported no financial conflicts of interest. There was no investigational or off-label use.

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There is no conflict of interest between authors.

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Authors and Affiliations

  1. Department of Physical Medicine and Rehabilitation Medical Faculty, Mersin University, Liparis Plaza-1 sitesi, H-Blok, 7/15, Mezitli, Mersin, Turkey

    Melek Sezgin & Günşah Şahin

  2. Department of Medical Biology and Genetics Medical Faculty, Mersin University, Mersin, Turkey

    İbrahim Ömer Barlas, Gözde Türköz & Mehmet Emin Erdal

  3. Department of Physical Medicine and Rehabilitation, Silifke State Hospital, Mersin, Turkey

    Seyfi Yıldır

  4. Department of Biostatistics Medical Faculty, Düzce University, Düzce, Turkey

    Handan Çamdeviren Ankaralı

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  1. Melek Sezgin
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  2. İbrahim Ömer Barlas
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  3. Seyfi Yıldır
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  4. Gözde Türköz
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  6. Günşah Şahin
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  7. Mehmet Emin Erdal
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Correspondence to Melek Sezgin.

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Sezgin, M., Barlas, İ.Ö., Yıldır, S. et al. Apoptosis-related Fas and FasL gene polymorphisms’ associations with knee osteoarthritis. Rheumatol Int 33, 2039–2043 (2013). https://doi.org/10.1007/s00296-013-2688-1

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  • DOI: https://doi.org/10.1007/s00296-013-2688-1

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