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In Vitro and In Vivo Assessment of Angiotensin-Converting Enzyme (ACE) Inhibitory Activity of Fermented Soybean Milk by Lactobacillus casei Strains

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Abstract

Angiotensin-converting enzyme (ACE) inhibitory activity of fermented soybean milk (FSM) by Lactobacillus casei strains in vitro was investigated in this study. Effects of fermented soybean milk administration by gavage on systolic blood pressure and diastolic blood pressure was also evaluated in spontaneously hypertensive rats (SHR) rats and Wistar-Kyoto (WKY) rats. Results showed that, CICC 20280 and CICC 23184 FSM showed high ACE inhibitory activity in vitro test and ACE inhibitory activity of CICC 23184 FSM was higher than CICC 20280 FSM. The bioactive substances of FSM were peptide and γ-aminobutyric acid (GABA). Their contents in CICC 20280 FSM and CICC 23184 FSM were 3.97 ± 0.67 mg/ml (peptide), 1.71 ± 0.36 mg/ml (GABA) and 5.17 ± 0.22 mg/ml (peptide), 1.57 ± 0.21 mg/ml (GABA), respectively. Moreover, CICC 20280 and CICC 23184 FSM administration by gavage could effectively lower the blood pressure of SHR to a normal level, while there was no effect on blood pressure of WKY rats. This result indicated that the bioactive substances could play an antihypertensive role when the blood pressure was not within the normal levels (high levels).

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Acknowledgments

This study was funded by Key Laboratory of Soybean Biology in Chinese Ministry of Education, Northeast Agricultural University (SB14C03) and Project of National Key Technology Research and Development Program for The 12th Five-year Plan (No. 2012BAD33B03)

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  1. Northeast Agricultural University, Harbin, 150030, Heilongjiang, China

    Zhijie Bao & Yujie Chi

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  1. Zhijie Bao
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  2. Yujie Chi
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Correspondence to Yujie Chi.

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The authors declare that they have no conflict of interest.

Research Involving Human and Animal Participants

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. This article does not contain any studies with human participants performed by any of the authors.

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Bao, Z., Chi, Y. In Vitro and In Vivo Assessment of Angiotensin-Converting Enzyme (ACE) Inhibitory Activity of Fermented Soybean Milk by Lactobacillus casei Strains. Curr Microbiol 73, 214–219 (2016). https://doi.org/10.1007/s00284-016-1051-7

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  • DOI: https://doi.org/10.1007/s00284-016-1051-7

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