Skip to main content

Advertisement

SpringerLink
Log in

Pseudomonas seleniipraecipitans Proteins Potentially Involved in Selenite Reduction

  • Published:
Current Microbiology Aims and scope Submit manuscript

Abstract

Pseudomonas seleniipraecipitans grows in the presence of high levels of selenite and selenate and reduces both oxyanions to elemental selenium (Se0), a property that may make P. seleniipraecipitans useful as an inoculant for biobarriers designed to remove selenite or selenate from ground or surface waters. An earlier study showed that P. seleniipraecipitans nitrate reductase reduced selenate to Se0, but failed to identify the protein(s) involved in selenite reduction. This study used ammonium sulfate precipitation, hydrophobic interaction chromatography, and native PAGE to isolate two electrophoretic gel regions, identified as bands A and B that showed selenite-reductase-activity. Proteomics was used to identify the proteins present in those regions. Glutathione reductase (GR) was detected in the A-band; based on this information, Saccharomyces cerevisiae GR, obtained from a commercial source, was evaluated and found to have selenite-reductase-activity, confirming that GR can reduce selenite to Se0. Proteomics was also used to detect the proteins present in the B-band and thioredoxin reductase (ThxR) was detected as a B-band protein; based on this information, E. coli ThxR, obtained from a commercial source, was evaluated and found to have selenite-reductase-activity, confirming that ThxR can reduce selenite to elemental selenium. Thus, evidence presented in this study shows that S. cerevisiae GR and E. coli ThxR can reduce SeO3 2− to Se0 and strongly suggests that P. seleniipraecipitans GR and ThxR can also reduce SeO3 2− to Se0.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Bibien M, Kirsch J, Mijean V, Vermiglio A (2002) Involvement of a putative molybdenum enzyme in the reduction of selenate by Escherichia coli. Microbiology 148:3865–3872

    Google Scholar 

  2. Combs GF Jr, Garbisu C, Yee BC et al (1996) Bioavailability of selenium accumulated by selenite-reducing bacteria. Biol Trace Elem Res 52:209–225

    Article  CAS  PubMed  Google Scholar 

  3. Dhillon KS, Dhillon SK (2003) Distribution and management of seleniferous soils. Adv Agronomy 79:119–184

    Article  CAS  Google Scholar 

  4. Dong Y, Zhang H, Hawthorn L, Ganther HE, Ip C (2003) Delineation of the molecular basis for selenium-induced growth arrest in human prostate cancer cells by oligonucleotide array. Cancer Res 63:52–59

    CAS  PubMed  Google Scholar 

  5. Eustice DC, Kull FJ, Shrift A (1981) Selenium toxicity: aminoacylation and peptide bond formation with selenomethionine. Plant Physiol 67:1954–1958

    Google Scholar 

  6. Euzéby J (2011) Validation List no. 140, List of new names and new combinations previously effectively, but not validly, published. Int J Sys Evol Microbiol 61:1499–1501

    Article  Google Scholar 

  7. GenomeNet. http://www.genome.jp/ Confirmed 27 Aug 2013

  8. Hirt RP, Müller S, Embley TM, Coombs GH (2002) The diversity and evolution of thioredoxin reductase: new perspectives. Trends Parasitol 18:302–308. doi:10.1016/S1471-4922(02)02293-6 PMID 12379950

    Article  CAS  PubMed  Google Scholar 

  9. Hunter WJ (2007) An Azospira oryzae (syn Dechlorosoma suillum) strain that reduces selenate and selenite to elemental red selenium. Curr Microbiol 54:376–381

    Article  CAS  PubMed  Google Scholar 

  10. Hunter WJ (2013) A Rhizobium selenitireducens protein showing selenite reductase activity. Curr Microbiol 68(3):311–316. doi:10.1007/s00284-013-0474-7

    Article  PubMed  Google Scholar 

  11. Hunter WJ, Kuykendall LD (2005) Removing selenite from groundwater with an in situ biobarrier: laboratory studies. Curr Microbiol 50:145–150

    CAS  PubMed  Google Scholar 

  12. Hunter WJ, Kuykendall LD (2007) Reduction of selenite to elemental red selenium by Rhizobium sp. strain B1. Curr Microbiol 55:344–349

    Article  CAS  PubMed  Google Scholar 

  13. Hunter WJ, Manter DK (2009) Reduction of selenite to elemental red selenium by Pseudomonas sp. strain CA5. Curr Microbiol 58:493–498

    Article  CAS  PubMed  Google Scholar 

  14. Hunter WJ, Manter DK (2011) Pseudomonas seleniipraecipitatus sp. nov.: a selenite reducing γ-proteobacteria isolated from soil. Curr Microbiol 62:565–569

    Article  CAS  PubMed  Google Scholar 

  15. Kessi J (2006) Enzymic systems proposed to be involved in the dissimilatory reduction of selenite in the purple non-sulfur bacteria Rhodospirillum rubrum and Rhodobacter capsulatus. Microbiology 152:731–743

    Article  CAS  PubMed  Google Scholar 

  16. MacFarquhar JK, Broussard DL, Melstrom P, Hutchinson R, Wolkin A, Martin C, Burk RF, Dunn JR, Green AL, Hammond R, Schaffner W, Jones TF (2010) Acute selenium toxicity associated with a dietary supplement. Arch Intern Med 170:256–261

    Article  PubMed Central  PubMed  Google Scholar 

  17. Pierru B, Grosse S, Pignol D, Sabaty M (2006) Genetic and biochemical evidence for the involvement of a molybdenum-dependent enzyme in one of the selenite reduction pathways of Rhodobacter sphaeroides f. sp. denitrificans IL106. Appl Environ Microbiol 72:3147–3153

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  18. Scott D, Toney M, Muzika M (2008) Harnessing the mechanism of glutathione reductase for synthesis of active site bound metallic nanoparticles and electrical connection to electrodes. J Am Chem Soc 130:865–874

    Article  CAS  PubMed  Google Scholar 

  19. Somogyi Z, Kádár I, Kiss I, Juríková T, Szekeres L, Balla S, Nagy P, Bakonyi (2012) Comparative toxicity of the selenate and selenite to the potworm Enchytraeus albidus (Annelida: Enchytraeidae) under laboratory conditions. Eur J Soil Biol 50:159–164

    Article  CAS  Google Scholar 

  20. Turner RJ, Weiner JH, Taylor DE (1998) Selenium metabolism in Escherichia coli. Biometals 11:223–227

    Article  CAS  PubMed  Google Scholar 

  21. USEPA (2003) Ground water and drinking water: list of drinking water contaminants and MCLs. http://www.epa.gov/safewater/mcl.html#inorganic (Confirmed May 16 2013)

  22. Watts CA, Ridley H, Condie KL, Leaver JT, Richardson DJ, Butler CS (2003) Selenate reduction by Enterobacter cloacae SLD1a-1 is catalysed by a molybdenum-dependent membrane-bound enzyme that is distinct from the membrane-bound nitrate reductase. FEMS Microbiol Lett 228:273–279

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

The author thanks Robin Montenieri and Joshua Padilla for their expert technical assistance. Manufacturer and product brand names are given for the reader’s convenience and do not reflect endorsement by the US government. This article was the work of US government employees engaged in their official duties and is exempt from copyright.

Author information

Authors and Affiliations

  1. United States Department of Agriculture–Agricultural Research Service, 2150-D Centre Avenue, Fort Collins, CO, 80526-8119, USA

    William J. Hunter

Authors
  1. William J. Hunter
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to William J. Hunter.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hunter, W.J. Pseudomonas seleniipraecipitans Proteins Potentially Involved in Selenite Reduction. Curr Microbiol 69, 69–74 (2014). https://doi.org/10.1007/s00284-014-0555-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00284-014-0555-2

Keywords

Search

Navigation