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Phylogenetic Groups Among Klebsiella pneumoniae Isolates from Brazil: Relationship with Antimicrobial Resistance and Origin

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Abstract

The objectives of this study were to determine the distribution of phylogenetic groups among Klebsiella pneumoniae isolates from Recife, Brazil and to assess the relationship between the groups and the isolation sites and resistance profile. Ninety four isolates of K. pneumoniae from hospital or community infections and from normal microbiota were analyzed by gyrA PCR–RFLP, antibiotic susceptibility, and adonitol fermentation. The results revealed the distinction of three phylogenetic groups, as it has also been reported in Europe, showing that these clusters are highly conserved within K. pneumoniae. Group KpI was dominantly represented by hospital and community isolates while groups KpII and KpIII displayed mainly normal microbiota isolates. The resistance to third generation cephalosporins, aztreonam, imipenem, amoxicillin/clavulanic acid, and streptomycin was only observed in KpI. The percentage of resistance was higher in KpI, followed by KpII and KpIII. The differences in the distribution of K. pneumoniae phylogenetic groups observed in this study suggest distinctive clinical and epidemiological characteristics among the three groups, which is important to understand the epidemiology of infections caused by this organism. This is the first study in Brazil on K. pneumoniae isolates from normal microbiota and community infections regarding the distribution of phylogenetic groups based on the gyrA gene.

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Acknowledgments

This work was financially supported by Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco (FACEPE).

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Authors and Affiliations

  1. Departamento de Medicina Tropical, Universidade Federal de Pernambuco, Recife, PE, 50.732-970, Brazil

    Maíra Espíndola Silva de Melo, Maria Amélia Vieira Maciel, Vera Magalhães da Silveira & Ana Catarina de Souza Lopes

  2. Laboratório de Imunopatologia Keizo Asami, Universidade Federal de Pernambuco, Recife, PE, 50.732-970, Brazil

    Maíra Espíndola Silva de Melo, Adriane Borges Cabral & Ana Catarina de Souza Lopes

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  1. Maíra Espíndola Silva de Melo
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  2. Adriane Borges Cabral
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  3. Maria Amélia Vieira Maciel
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  4. Vera Magalhães da Silveira
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  5. Ana Catarina de Souza Lopes
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Correspondence to Ana Catarina de Souza Lopes.

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de Melo, M.E.S., Cabral, A.B., Maciel, M.A.V. et al. Phylogenetic Groups Among Klebsiella pneumoniae Isolates from Brazil: Relationship with Antimicrobial Resistance and Origin. Curr Microbiol 62, 1596–1601 (2011). https://doi.org/10.1007/s00284-011-9903-7

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  • DOI: https://doi.org/10.1007/s00284-011-9903-7

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