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Isolation and Identification of a Novel Strain of Pseudomonas chlororaphis Capable of Transforming Isoeugenol to Vanillin†

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Abstract

Vanillin is undoubtedly one of the most popular and widely used flavoring agents in the world. Taking into consideration the worldwide demand for natural vanillin and its limited supply, alternative routes for its production including biotransformation are being constantly explored. In this regard, a novel soil bacterium capable of converting isoeugenol to vanillin was isolated by conventional enrichment process from soils of Ocimum field. On the basis of morphological and physiochemical characteristics and 16S rRNA gene sequence analysis, the isolate was identified as Pseudomonas chlororaphis CDAE5 (EMBL # AM158279). Vanillin formation was analyzed by gas chromatography (GC), and its structure was confirmed by GC-mass spectrometry and nuclear magnetic resonance. After 24-h reaction, the vanillin concentration reached 1.2 g L−1 from 10 g L−1 isoeugenol in 20-mL reaction solution at 25°C and 180 rpm. The strain showed potential to be a good candidate for biotechnological production of vanillin from isoeugenol. Further studies for standardization and optimization for higher yield of vanillin production needs to be investigated.

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Acknowledgments

This study was supported by a project (SMM-0002) sponsored by Council of Scientific & Industrial Research, New Delhi, India. The authors gratefully acknowledge the Director, IHBT, Palampur, for providing the necessary facilities during the course of the project.

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Authors and Affiliations

  1. Hill Area Tea Science Division, Institute of Himalayan Bioresource Technology, Post Box No. 6, Palampur, 176061, Himachal Pradesh, India

    Ramesh C. Kasana

  2. Natural Plant Products Division, Institute of Himalayan Bioresource Technology, Post Box No. 6, Palampur, 176061, Himachal Pradesh, India

    Upendra K. Sharma, Nandini Sharma & Arun K. Sinha

Authors
  1. Ramesh C. Kasana
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  2. Upendra K. Sharma
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  3. Nandini Sharma
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  4. Arun K. Sinha
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Corresponding author

Correspondence to Arun K. Sinha.

Additional information

†IHBT Communication No. 0676

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Kasana, R.C., Sharma, U.K., Sharma, N. et al. Isolation and Identification of a Novel Strain of Pseudomonas chlororaphis Capable of Transforming Isoeugenol to Vanillin† . Curr Microbiol 54, 457–461 (2007). https://doi.org/10.1007/s00284-006-0627-z

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  • DOI: https://doi.org/10.1007/s00284-006-0627-z

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