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Genome engineering in actinomycetes using site-specific recombinases

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Abstract

The rational modification of the actinomycetes genomes has a variety of applications in research, medicine, and biotechnology. The use of site-specific recombinases allows generation of multiple mutations, large DNA deletions, integrations, and inversions and may lead to significant progress in all of these fields. Despite their huge potential, site-specific recombinase-based technologies have primarily been used for simple marker removal from a chromosome. In this review, we summarise the site-specific recombination approaches for genome engineering in various actinomycetes.

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Acknowledgments

The work in the laboratory of AL was supported by the BMBF (GenBioCom), DFG (Lu1524/2-1) and ERC (EXPLOGEN) grants.

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  1. Helmholtz-Institute for Pharmaceutical Research Saarland, Campus, Building C2.3, Saarbrücken, 66123, Germany

    Maksym Myronovskyi & Andriy Luzhetskyy

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  1. Maksym Myronovskyi
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  2. Andriy Luzhetskyy
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Correspondence to Andriy Luzhetskyy.

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Myronovskyi, M., Luzhetskyy, A. Genome engineering in actinomycetes using site-specific recombinases. Appl Microbiol Biotechnol 97, 4701–4712 (2013). https://doi.org/10.1007/s00253-013-4866-1

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