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Zinc oxide-doped poly(urethane) spider web nanofibrous scaffold via one-step electrospinning: a novel matrix for tissue engineering

  • Applied Microbial and Cell Physiology
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Abstract

Zinc oxide (ZnO) nanostructures have been commonly studied for electronic purposes due to their unique piezoelectric and catalytic properties; however, recently, they have been also exploited for biomedical applications. The purpose of this study was to fabricate ZnO-doped poly(urethane) (PU) nanocomposite via one-step electrospinning technique. The utilized nanocomposite was prepared by using colloidal gel composed of ZnO and PU, and the obtained mats were vacuum dried at 60 °C overnight. The physicochemical characterization of as-spun composite nanofibers was carried out by X-ray diffraction pattern, field emission scanning electron microscopy, energy-dispersive X-ray spectroscopy, electron probe microanalysis, and transmission electron microscopy, whereas the thermal behavior was analyzed by thermogravimetric analysis. The viability, attachment, and proliferation of NIH 3T3 mouse fibroblast cells on the ZnO/PU composite nanofibers were analyzed by in vitro cell compatibility test. The morphological features of the cells attached on nanofibers were examined by Bio-SEM. We conclude that the electrospun nanofibrous scaffolds with unique spider nets had good biocompatibility. Cytotoxicity experiments indicated that the mouse fibroblasts could attach to the nanocomposite after being cultured. Thus, the current work demonstrates that the as-synthesized ZnO/PU hybrid nanofibers represent a promising biomaterial to be exploited for various tissue engineering applications.

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Acknowledgments

This work has been conducted and partly supported by a grant from Next-Generation BioGreen 21 Program (no. PJ008191) and a fund for FTA issues (no. PJ907055), Rural Development Administration, Republic of Korea.

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Authors and Affiliations

  1. Department of Animal Science and Institute of Rare Earth for Biological Application, Chonbuk National University, Jeonju, 561-756, Republic of Korea

    Touseef Amna & I. H. Hwang

  2. Department of Organic Materials and Fiber Engineering, Chonbuk National University, Jeonju, 561-756, Republic of Korea

    M. Shamshi Hassan

  3. Department of Chemistry, University of Texas–Pan American, Edinburg, TX, 78539, USA

    Faheem A. Sheikh

  4. Genomic Informatics Center, Hankyong National University, Anseong, Republic of Korea

    Hak Kyo Lee

  5. Department of Animal Science and Technology, Sunchon National University, Suncheon, 540-742, Republic of Korea

    Kang-Seok Seo

  6. Department of Animal Science, College of Ecological and Environmental Science, Kyungpook National University, Sangju, 742-711, Republic of Korea

    Duhak Yoon

Authors
  1. Touseef Amna
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  2. M. Shamshi Hassan
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  3. Faheem A. Sheikh
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  4. Hak Kyo Lee
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  5. Kang-Seok Seo
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  6. Duhak Yoon
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  7. I. H. Hwang
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Correspondence to Touseef Amna or I. H. Hwang.

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Amna, T., Hassan, M.S., Sheikh, F.A. et al. Zinc oxide-doped poly(urethane) spider web nanofibrous scaffold via one-step electrospinning: a novel matrix for tissue engineering. Appl Microbiol Biotechnol 97, 1725–1734 (2013). https://doi.org/10.1007/s00253-012-4353-0

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  • DOI: https://doi.org/10.1007/s00253-012-4353-0

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