Abstract
To enhance the potential therapeutic efficacy of an antimicrobial peptide human β-defensin 3, two fusion peptides, a bactericidal–immunomodulatory fusion peptide human β-defensin 3-mannose-binding lectin and a bactericidal–bactericidal fusion peptide human β-defensin 3-lysozyme were synthesized and the bactericidal activities in vitro and in vivo against methicillin-resistant Staphylococcus aureus N315 were demonstrated in this study. Peptide human β-defensin 3-lysozyme showed the best bactericidal activity in vitro, but human β-defensin 3-mannose-binding lectin showed a significant improvement in angiogenesis and tissue reconstruction. Our results illustrated that outstanding bactericidal activity in vitro is not essential in the development of antimicrobial peptides. Fusion strategy and immunomodulatory factors should be utilized in novel antimicrobial peptide development.
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Acknowledgments
The authors thank Aifen Fu for her technical expertise. Research is supported in part by the National Natural Science Foundation of China (number 30830002, number 30670102, and number 30770820), the National Key Program for Infectious Diseases of China (number 2008ZX10004-002, number 2008ZX10004-009, and number 2009ZX10004-712), Program of Shanghai Subject Chief Scientist (number 09XD1402700), and the National High Technology Research and Development Program of China.
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Li, Q., Zhou, Y., Dong, K. et al. Potential therapeutic efficacy of a bactericidal–immunomodulatory fusion peptide against methicillin-resistant Staphylococcus aureus skin infection. Appl Microbiol Biotechnol 86, 305–309 (2010). https://doi.org/10.1007/s00253-009-2313-0
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DOI: https://doi.org/10.1007/s00253-009-2313-0