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Characterization and Identification of Recalcitrant Organic Pollutants (ROPs) in Tannery Wastewater and Its Phytotoxicity Evaluation for Environmental Safety

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Abstract

Tannery wastewater (TWW) is of serious environmental concern to pollution control authorities, because it contains highly toxic, recalcitrant organic and inorganic pollutants. The nature and characteristics of recalcitrant organic pollutants (ROPs) are not fully explored to date. Hence, the purpose of this study was to characterize and identify the ROPs present in the treated TWW. Gas chromatography–mass spectrometry data analysis showed the presence of a variety of ROPs in the treated TWW. Results unfolded that benzyl chloride, butyl octyl phthalate, 2,6-dihydroxybenzoic acid 3TMS, dibutyl phthalate, benzyl alcohol, benzyl butyl phthalate, 4-chloro-3-methyl phenol, phthalic acid, 2′6′-dihydroxyacetophenone, diisobutyl phthalate, 4-biphenyltrimethylsiloxane, di-(-2ethy hexyl)phthalate, 1,2-benzenedicarboxylic acid, dibenzyl phthalate, and nonylphenol were present in the treated TWW. Due to endocrine disrupting nature and aquatic toxicity, the U.S. Environmental Protection Agency classified many of these as “priority pollutants” and restricted their use in leather industries. In addition, the physicochemical analysis of the treated TWW also showed very high BOD, COD, and TDS values along with high Cr and Pb content beyond the permissible limits for industrial discharge. Furthermore, phytotoxicity assessment unfolds the inhibitory effects of TWW on the seed germination, seedling growth parameters, and α-amylase activity in Phaseolus aureus L. This indicates that the TWW discharged even after secondary treatment into the environment has very high pollution parameters and may cause a variety of serious health threats in living beings upon exposure. Overall, the results reported in this study will be helpful for the proper treatment and management of TWW to combat the environmental threats.

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Acknowledgements

This work was financially supported by “Major Research Project” (Grant No.: SB/EMEQ-357/2013) from “Science and Engineering Research Board” (SERB), Department of Science & Technology (DST), Government of India (GOI), New Delhi, India, and University Grant Commission (UGC) Fellowship received by Mr. Gaurav Saxena for doctoral research work is duly acknowledged. The authors also are thankful to Dr. Subhash Awasthi, In-charge CETP, Unnao for kindly providing the TWW samples, Director, University Science Instrumentation Centre (USIC), BBAU for FT-IT analysis, Head, Department of Applied Statistics, BBAU for statistical analysis, and anonymous reviewers for their valuable comments.

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Authors and Affiliations

  1. Laboratory for Bioremediation and Metagenomics Research (LBMR), Department of Environmental Microbiology (DEM), Babasaheb Bhimrao Ambedkar University (A Central University), Vidya Vihar, Raebareli Road, Lucknow, Uttar Pradesh, 226 025, India

    Ram Naresh Bharagava & Gaurav Saxena

  2. Key Laboratory of Urban Environment and Health, Institute of Urban Environment (IUE), Chinese Academy of Sciences (CAS), Xiamen, 361021, People’s Republic of China

    Sikandar I. Mulla

  3. Analytical Chemistry Division and Regulatory Toxicology Group, CSIR-Indian Institute of Toxicology Research, Vishvigyan Bhawan 31, Mahatma Gandhi Marg, Lucknow, Uttar Pradesh, 226 001, India

    Devendra Kumar Patel

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  1. Ram Naresh Bharagava
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Correspondence to Ram Naresh Bharagava.

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Bharagava, R.N., Saxena, G., Mulla, S.I. et al. Characterization and Identification of Recalcitrant Organic Pollutants (ROPs) in Tannery Wastewater and Its Phytotoxicity Evaluation for Environmental Safety. Arch Environ Contam Toxicol 75, 259–272 (2018). https://doi.org/10.1007/s00244-017-0490-x

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