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Synthetic Pyrethroid Cypermethrin Induced Cellular Damage in Reproductive Tissues of Drosophila melanogaster: Hsp70 as a Marker of Cellular Damage

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Abstract

We tested a working hypothesis of whether the synthetic pyrethroid cypermethrin, used worldwide for insecticidal purpose, causes adverse effects on reproduction in Drosophila melanogaster. Freshly eclosed first instar larvae of a transgenic strain of Drosophila melanogaster, Bg 9, transgenic for hsp70 (hsp70-lacZ), were transferred to different dietary concentrations of the test chemical (0.002, 0.02, 0.2, 0.5, and 50.0 ppm). Larval mortality was observed at the higher dosed groups (0.2, 0.5, and 50.0 ppm). Following pair mating of virgin flies emerging from the treatment groups, a significant (p < 0.05) effect on reproduction was observed in the lowest two dietary concentrations of the test chemical as compared to control. The test chemical exhibited a hazardous effect on the reproductive organs of the exposed organism as evident by Hsp70 expression and tissue damage. The impact of damage was comparatively more prominent in male flies than in females. Hsp70 expression was restricted only within the testis lobes of male, while ovary in the female fly did not exhibit any Hsp70 expression. Interestingly, the accessory glands of male flies in these treatment groups reflected intense tissue damage as evident by Trypan Blue staining. This was further corroborated by ultrastructural changes like higher vacuolization and disorganized filamentous bodies in the accessory glands of these groups. The present study indicates a profound effect on reproduction by cypermethrin and suggests the protective role of hsp70.

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Acknowledgments

The authors are thankful to the Director, ITRC, for his support and interest throughout the study. The authors wish to thank Prof. S.C. Lakhotia, Cytogenetics Laboratory, Banaras Hindu University, Varanasi, for the Bg 9 fly strain, Prof. Susan Lindquist, Whitehead Institute, Cambridge, MA, and Prof. M.B. Evgenev, Engelhardt Institute of Molecular Biology, Moscow, Russia, for Drosophila Hsp70 monoclonal antibody (7Fb). M/S Aimco Pesticides India Ltd., India, is acknowledged for providing the technical grade cypermethrin. I.M. and H.R.S. were supported by the Council of Scientific and Industrial Research Senior Research Fellowships. Financial assistance from the Council of Scientific and Industrial Research and the Department of Biotechnology, Govt. of India, New Delhi (Grant No. BT/PRO 390/R&D/12/030/96), to D.K.C. is gratefully acknowledged. This report is ITRC communication no. 2146.

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  1. Indranil Mukhopadhyay & Debapratim Kar Chowdhuri

    Present address: Laboratory of Clinical Trials Unit, Developmental Therapeutics Program, National Cancer Institute, National Institutes of Health, Bldg 37, Rm 1052, 9000 Rockville Pike, Bethesda, MD, India

Authors and Affiliations

  1. Embryotoxicology Section, Industrial Toxicology Research Centre, P.O. Box No. 80, M.G. Marg, Lucknow, 226 001, Uttar Pradesh, India

    Indranil Mukhopadhyay, Hifzur Rahman Siddique, Daya Krishna Saxena & Debapratim Kar Chowdhuri

  2. Electron Microscopy Unit, Central Drug Research Institute, Chattar Manzil, M.G. Marg, Lucknow, 226 001 , Uttar Pradesh, India

    Virendra Kumar Bajpai

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  1. Indranil Mukhopadhyay
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Correspondence to Debapratim Kar Chowdhuri.

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Mukhopadhyay, I., Siddique, H.R., Bajpai, V.K. et al. Synthetic Pyrethroid Cypermethrin Induced Cellular Damage in Reproductive Tissues of Drosophila melanogaster: Hsp70 as a Marker of Cellular Damage. Arch Environ Contam Toxicol 51, 673–680 (2006). https://doi.org/10.1007/s00244-005-0169-6

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