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Imaging of lipids in rat heart by MALDI-MS with silver nanoparticles

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Abstract

Lipids are a major component of heart tissue and perform several important functions such as energy storage, signaling, and as building blocks of biological membranes. The heart lipidome is quite diverse consisting of glycerophospholipids such as phosphatidylcholines (PCs), phosphatidylethanolamines (PEs), phosphatidylinositols (PIs), phosphatidylglycerols (PGs), cardiolipins (CLs), and glycerolipids, mainly triacylglycerols (TAGs). In this study, mass spectrometry imaging (MSI) enabled by matrix implantation of ionized silver nanoparticles (AgNP) was used to map several classes of lipids in heart tissue. The use of AgNP matrix implantation was motivated by our previous work showing that implantation doses of only 1014/cm2 of 2 nm gold nanoparticulates into the first 10 nm of the near surface of the tissue enabled detection of most brain lipids (including neutral lipid species such as cerebrosides) more efficiently than traditional organic MALDI matrices. Herein, a similar implantation of 500 eV AgNP− across the entire heart tissue section results in a quick, reproducible, solvent-free, uniform matrix concentration of 6 nm AgNP residing near the tissue surface. MALDI-MSI analysis of either positive or negative ions produce high-quality images of several heart lipid species. In negative ion mode, 24 lipid species [16 PEs, 4 PIs, 1 PG, 1 CL, 2 sphingomyelins (SMs)] were imaged. Positive ion images were also obtained from 29 lipid species (10 PCs, 5 PEs, 5 SMs, 9 TAGs) with the TAG species being heavily concentrated in vascular regions of the heart.

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Acknowledgments

This research was supported by the Intramural Research Program of the National Institute on Drug Abuse, NIH. The authors acknowledge Dr. Mari Prieto and Thermo Fisher Corporation for technical and instrumentation advices. Ionwerks and the University of Pittsburgh gratefully acknowledge ARRA support through NIDA phase II SBIR grant 1RC3DA031431-01.

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Authors and Affiliations

  1. Structural Biology Unit, NIDA IRP, NIH, 333 Cassell Drive, Room 1120, Baltimore, MD, 21224, USA

    Shelley N. Jackson, Kathrine Baldwin, Ludovic Muller, Virginia M. Womack & Amina S. Woods

  2. University of Pittsburgh, 4200 Fifth Ave, Pittsburgh, PA, 15213, USA

    Ludovic Muller, Virginia M. Womack & Carey Balaban

  3. Ionwerks, Inc., 3401 Louisiana, Suite 355, Houston, TX, 77002, USA

    J. Albert Schultz

Authors
  1. Shelley N. Jackson
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  2. Kathrine Baldwin
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  3. Ludovic Muller
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  4. Virginia M. Womack
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  5. J. Albert Schultz
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  6. Carey Balaban
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  7. Amina S. Woods
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Corresponding author

Correspondence to Amina S. Woods.

Additional information

Published in the topical collection Biomedical Mass Spectrometry with guest editors Mitsutoshi Setou, Toshimitsu Niwa, and Akira Ishii.

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Jackson, S.N., Baldwin, K., Muller, L. et al. Imaging of lipids in rat heart by MALDI-MS with silver nanoparticles. Anal Bioanal Chem 406, 1377–1386 (2014). https://doi.org/10.1007/s00216-013-7525-6

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  • DOI: https://doi.org/10.1007/s00216-013-7525-6

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