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Direct analysis in real time mass spectrometry combined with single-drop liquid–liquid–liquid microextraction for the rapid analysis of multiple phytohormones in fruit juice

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Abstract

A rapid, simple, and efficient method for the fast determination of multiple phytohormones was developed in this work, based on single-drop liquid–liquid–liquid microextraction (SD-LLLME) combined with direct analysis in real-time mass spectrometry (DART-MS). Six phytohormones—indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), jasmonic acid (JA), salicylic acid (SA), abscisic acid (ABA), and gibberellin A3 (GA3)—were analyzed simultaneously using this method, and the conditions employed for DART-MS and SD-LLLME were optimized systematically. Satisfactory results were obtained in terms of linearity (R 2 values for all phytohormones were 0.991–0.996), sensitivity (limits of detection were 0.65–72 ng/mL), and repeatability (RSD values were 6.9–14%). In addition, the proposed method was applied to determine the endogenous phytohormones in three kinds of fruit juice. Different concentrations of phytohormones were detected with satisfactory recoveries, and the whole analytical procedure took no more than 30 min. Therefore, this combination of SD-LLLME and DART-MS was shown to be a suitable and effective approach for the fast analysis of targets present at trace level concentrations in complex matrices.

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Acknowledgement

This research was supported by the National Natural Science Foundation of China (grants 90717002, 20905005, 20115008, and 20805001), and by the fundamental research funds for the central universities.

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Authors and Affiliations

  1. Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China

    Yu Bai, Jialing Zhang, Yu Bai & Huwei Liu

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  1. Yu Bai
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  2. Jialing Zhang
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  3. Yu Bai
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  4. Huwei Liu
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Corresponding author

Correspondence to Yu Bai.

Additional information

Published in the special issue Young Investigators in Analytical and Bioanalytical Science with guest editors S. Daunert, J. Bettmer, T. Hasegawa, Q. Wang and Y. Wei.

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Bai, Y., Zhang, J., Bai, Y. et al. Direct analysis in real time mass spectrometry combined with single-drop liquid–liquid–liquid microextraction for the rapid analysis of multiple phytohormones in fruit juice. Anal Bioanal Chem 403, 2307–2314 (2012). https://doi.org/10.1007/s00216-012-5728-x

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  • DOI: https://doi.org/10.1007/s00216-012-5728-x

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