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Microchip-based cellular biochemical systems for practical applications and fundamental research: from microfluidics to nanofluidics

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Abstract

By combining cell technology and microchip technology, innovative cellular biochemical tools can be created from the microscale to the nanoscale for both practical applications and fundamental research. On the microscale level, novel practical applications taking advantage of the unique capabilities of microfluidics have been accelerated in clinical diagnosis, food safety, environmental monitoring, and drug discovery. On the other hand, one important trend of this field is further downscaling of feature size to the 101–103 nm scale, which we call extended-nano space. Extended-nano space technology is leading to the creation of innovative nanofluidic cellular and biochemical tools for analysis of single cells at the single-molecule level. As a pioneering group in this field, we focus not only on the development of practical applications of cellular microchip devices but also on fundamental research to initiate new possibilities in the field. In this paper, we review our recent progress on tissue reconstruction, routine cell-based assays on microchip systems, and preliminary fundamental method for single-cell analysis at the single-molecule level with integration of the burgeoning technologies of extended-nano space.

By combination of cell technology and microchip technology, innovative cellular biochemical tools can be created from the microscale to the nanoscale, for both practical applications and fundamental research. The image demonstrates a concept of analysis of a single cell at the singlemolecule level on a microchip with integration of extended-nano (101–103 nm) space.

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Acknowledgement

This work was partially supported by the JSPS Core-to-Core Program and SCF (Special Coordination Funds for Promoting Science and Technology) of MEXT of Japan.

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Authors and Affiliations

  1. Department of Applied Chemistry, School of Engineering, The University of Tokyo, 7-3-1, Hongo, Bunkyo, Tokyo, 113-8656, Japan

    Yan Xu, Kihoon Jang, Yo Tanaka, Kazuma Mawatari & Takehiko Kitamori

  2. Nanoscience and Nanotechnology Research Center, Research Organization for the 21st Century, Osaka Prefecture University, 1-2, Gakuen-cho, Naka-ku, Sakai, Osaka, 599-8570, Japan

    Yan Xu

  3. Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency, 1-1-2, Otemachi, Chiyoda, Tokyo, 100-0004, Japan

    Yan Xu, Kihoon Jang, Yo Tanaka, Kazuma Mawatari & Takehiko Kitamori

  4. Department of Bioengineering, School of Engineering, The University of Tokyo, 7-3-1, Hongo, Bunkyo, Tokyo, 113-8656, Japan

    Tadahiro Yamashita, Kazuma Mawatari & Takehiko Kitamori

Authors
  1. Yan Xu
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  2. Kihoon Jang
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  3. Tadahiro Yamashita
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  4. Yo Tanaka
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  5. Kazuma Mawatari
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  6. Takehiko Kitamori
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Corresponding authors

Correspondence to Yan Xu or Takehiko Kitamori.

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Published in the 10th Anniversary Issue.

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Xu, Y., Jang, K., Yamashita, T. et al. Microchip-based cellular biochemical systems for practical applications and fundamental research: from microfluidics to nanofluidics. Anal Bioanal Chem 402, 99–107 (2012). https://doi.org/10.1007/s00216-011-5296-5

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  • DOI: https://doi.org/10.1007/s00216-011-5296-5

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