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Fluorescence polarization immunoassays and related methods for simple, high-throughput screening of small molecules

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Abstract

Fluorescence polarization immunoassay (FPIA) is a homogeneous (without separation) competitive immunoassay method based on the increase in fluorescence polarization (FP) of fluorescent-labeled small antigens when bound by specific antibody. The minimum detectable quantity of FPIAs with fluorescein label (about 0.1 ng analyte) is comparable with chromatography and ELISA methods, although this may be limited by sample matrix interference. Because of its simplicity and speed, FPIA is readily automated and therefore suitable for high-throughput screening (HTS) in a variety of application areas. Systems that involve binding of ligands to receptor proteins are also susceptible to analysis by analogous FP methods employing fluorescent-labeled ligand and HTS applications have been developed, notably for use in candidate drug screening.

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References

  1. Dandliker WB, Kelly RJ, Dandliker J, Farquhar J, Levin J (1973) Immunochemistry 10:219–227

    CAS  Google Scholar 

  2. Dandliker WB, Hsu M, Levin J, Rao BR (1981) Methods Enzymol 74:3–28

    Article  CAS  Google Scholar 

  3. Lakowicz JR (1983) Principles of fluorescence spectroscopy. Plenum, New York

    Google Scholar 

  4. Gutierrez MC, Gomez-Hens A, Peres-Bendito D (1989) Talanta 36:1187–1201

    CAS  Google Scholar 

  5. Rhys-Williams AT, Smith DS (1993) Fluorescence polarisation immunoassay. In: Albert WHW, Staines NA (eds) Methods of immunological analysis, fundamentals. VCH, Weinheim

    Google Scholar 

  6. Jolley ME, Stroupe SD, Wang CJ (1981) Clin Chem 27:1190–1197

    CAS  Google Scholar 

  7. Eremin SA (1995) Polarization fluoroimmunoassay for rapid, specific detection of pesticides. In: Nelson JO, Karu AE, Wong R (eds) Immunoanalysis of agrochemicals: emerging technologies. American Chemical Society, Washington

    Google Scholar 

  8. Jolley ME (1981) J Anal Toxicol 5:236–240

    CAS  Google Scholar 

  9. Jones AW (2004) J Anal Toxicol 28:541–546

    CAS  Google Scholar 

  10. Shim WB, Kolosova AY, Kim YJ, Yang ZY, Park SJ, Eremin SA, Lee IS, Chung DH (2004) Int J Food Sci Technol 39:829–837

    CAS  Google Scholar 

  11. Eremin SA (1998) Food Technol Biotechnol 36:235–243

    CAS  Google Scholar 

  12. Nasir MS, Jolley ME (1999) Comb Chem High Throughput Screening 2:177–190

    CAS  Google Scholar 

  13. Nasir MS, Jolley ME (2003) Comb Chem High Throughput Screening 6:267–273

    CAS  Google Scholar 

  14. Eremin SA, Smith DS (2003) Comb Chem High Throughput Screening 6:257–266

    CAS  Google Scholar 

  15. Dandliker WB, Halber SP, Schapiro HC (1965) J Exp Med 122:1029–1048

    CAS  Google Scholar 

  16. Watson RAA, Landon J, Shaw EJ, Smith DS (1976) Clin Chim Acta 73:51–55

    CAS  Google Scholar 

  17. McGregor AR, Crookall-Greening JO, Landon J, Smith DS (1978) Clin Chim Acta 83:162–166

    Google Scholar 

  18. Eremin SA, Gallacher G, Lotey H, Smith DS, Landon J (1987) Clin Chem 33:1903–1906

    CAS  Google Scholar 

  19. Colbert DL, Eremin SA, Landon J (1991) J Immunol Methods 140:227–233

    CAS  Google Scholar 

  20. Choi MJ, Choi J, Park J, Eremin SA (1995) J Immunoassay 16:263–278

    CAS  Google Scholar 

  21. Eremin SA, Smirnov AV, Gallacher G, Smith DS, Colbert DL (1993) Analyst 118:1325–1328

    CAS  Google Scholar 

  22. Eremin SA, Ufimtcheva EV, Izotov BN (1995) Voprosy narkologii 3:31–36 (in Russian)

    Google Scholar 

  23. Eremin SA (1995) Polarization fluoroimmunoassay for rapid, specific detection of pesticides. In: Nelson JO, Karu AE, Wong R (eds) Immunoanalysis of agrochemicals: emerging technologies. American Chemical Society, Washington

    Google Scholar 

  24. Eremin SA, Coxon RE, Colbert DL, Landon J, Smith DS (1992) Analyst 117:697–699

    CAS  Google Scholar 

  25. Mannaert E, Daenens P (1996) Analyst 121:857–861

    CAS  Google Scholar 

  26. Kada G, Kaiser K, Falk H, Gruber HJ (1999) Biochim Biophys Acta 1427:44–48

    CAS  Google Scholar 

  27. Adamzcyk M, Fishpaugh JR, Harrington CA, Orsulak P, Akers L (1994) Ther Drug Monit 16:577–587

    Google Scholar 

  28. Adamzcyk M, Fishpaugh JR, Harrington CA, Hartter DE, Vanderbilt AS, Orsulak P, Akers L (1994) Ther Drug Monit 16:298–311

    Article  Google Scholar 

  29. Kolosova AY, Eremin SA, Gavrilova EM, Egorov AM (1994) Problemy of endocrinology 4:48–51 (in Russian)

    Google Scholar 

  30. Choi MJ, Choi J, Yoon DY, Park J, Eremin SA (1997) Biol Pharm Bull 20:309–314

    CAS  Google Scholar 

  31. Adamczyk M, Chen YY, Johnson DD, Reddy RE (1997) Tetrahedron 53:12855–12866

    CAS  Google Scholar 

  32. Samsonova ZV, Eremin SA, Egorov AM, Franek M (1994) Bioorganicheskaya Khimiya 20:1359–1364 (in Russian) [Russ J Bioorg Chem (Engl Transl) 20:773–777]

    CAS  Google Scholar 

  33. Hatzidakis GI, Tsatsakis AM, Krambovitis EK, Spyros A, Eremin SA (2002) Anal Chem 74:2513–2521

    CAS  Google Scholar 

  34. Eremin SA, Melnichenko OA, Tumanov AA, Sorokina NV, Molokova EV, Egorov AM (1994) Voprosy meditsinskoi khimii 40:57–60 (in Russian)

    CAS  Google Scholar 

  35. Eremin SA, Egorov AM, Melnichenko OA, Tumanov AA (1995) Zh Anal Khim 50:215–218 (in Russian) [Russ J Anal Chem (Engl Transl) 50:198–201]

    Google Scholar 

  36. Eremin SA, Krikunova VS, Krasnova AI, Popova VA, Onnerfjord P (1998) Agrokhimiya (in Russian) 6:80–85. [Russian journal of Agrochemistry]

    Google Scholar 

  37. Krikunova VS, Eremin SA, Smith DS, Landon J (2003) Int J Environ Anal Chem 83:585–595

    CAS  Google Scholar 

  38. Polak IM, Eremin SA, Wilmer M, Renneberg R, Spener F (1994) Agrokhimiya 9:131–136 (in Russian)

    Google Scholar 

  39. Krikounova VS, Abuknesha R, Eremin SA (2002) The Scientific World Journal 2:1132–1137

    CAS  Google Scholar 

  40. Eremin SA, Bochkareva AE, Popova VA, Abad A, Manclus JJ, Mercader JV, Montoya A (2002) Anal Lett 35:1835–1850

    CAS  Google Scholar 

  41. Krasnova AI, Eremin SA, Natangelo M, Tavazzi S, Benfenati E (2001) Anal Lett 34:2285–2301

    CAS  Google Scholar 

  42. Eremin SA, Mel’nichenko OA, Kreissing S, Hock B (1995) Zh Anal Khim 50:971–978 (in Russian) [Russ J Anal Chem (Engl Transl) 50:888–895]

    Google Scholar 

  43. Mel’nichenko OA, Eremin SA, Egorov AM, Tumanov AA (1995) Vopr Med Khim 41:60–62 (in Russian). PMID: 7793102

    CAS  Google Scholar 

  44. Eremin SA, Ryabova IA, Yakovleva JN, Yazynina EV, Zherdev AV, Dzantiev BB (2002) Anal Chim Acta 468:229–236

    CAS  Google Scholar 

  45. Yakovleva J, Knopp D, Niessner R, Eremin SA (2002) Food Agric Immunol 14:217–229

    CAS  Google Scholar 

  46. Yakovleva JN, Lobanova AI, Panchenko OA, Eremin SA (2002) Int J Environ Anal Chem 82:851–863

    CAS  Google Scholar 

  47. Deryabina MA, Yakovleva YN, Popova VA, Eremin SA (2005) J Anal Chem 60:80–85 [Translated from Zhurnal Analiticheskoi Khimii 60:91–96]

    CAS  Google Scholar 

  48. Kolosova AY, Park JH, Eremin SA, Kang SJ, Chung DH (2003) J Agric Food Chem 51:1107–1114

    CAS  Google Scholar 

  49. Yakovleva J, Zeravik J, Michura IV, Formanovsky AA, Franek M, Eremin SA (2003) Int J Environ Anal Chem 83:597–607

    CAS  Google Scholar 

  50. Yakovleva JN, Lobanova AY, Shutaleva EA, Kourkina MA, Mart’ianov AA, Zherdev AV, Dzantiev BB, Eremin SA (2004) Anal Bioanal Chem 378:634–641

    CAS  Google Scholar 

  51. Fránek M, Zeravík J, Eremin SA, Yakovleva J, Badea M, Danet A, Nistor C, Ocio N, Emnéus J (2001) Fresenius J Anal Chem 371:456–466

    Google Scholar 

  52. Yakovleva J, Lobanova AY, Michura I, Formanovsky A, Franek M, Zeravik J, Eremin SA (2002) Anal Lett 35:2279–2294

    CAS  Google Scholar 

  53. Eremin SA, Knopp D, Niessner R, Hong JY, Park SJ, Choi MJ (2005) Environ Chem 2:227–234

    CAS  Google Scholar 

  54. Goryacheva IY, Shutaleva EA, Suchanek M, Niessner R, Knopp D, Eremin SA (2007) Anal Lett 40:1445–1460

    CAS  Google Scholar 

  55. Nasir MS, Jolley ME (2002) J Agric Food Chem 50:3116–3121

    CAS  Google Scholar 

  56. Maragos CM, Jolley ME, Nasir MS (2002) Food Addit Contam 19:400–407

    CAS  Google Scholar 

  57. Maragos CM, Plattner RD (2002) J Agric Food Chem 50:1827–1832

    CAS  Google Scholar 

  58. Lippolis V, Pascale M, Visconti A (2006) J Food Prot 69:2712–2719

    CAS  Google Scholar 

  59. Maragos CM, Jolley ME, Plattner RD, Nasir MS (2001) J Agric Food Chem 49:596–602

    CAS  Google Scholar 

  60. Maragos CM, Kim EK (2004) J Food Prot 67:1039–1043

    CAS  Google Scholar 

  61. Furzer GS, Veldhuis L, Hall JC (2006) J Agric Food Chem 54:688–693

    CAS  Google Scholar 

  62. Bicamumpaka C, Pag M (1998) J Immunol Methods 212:1–7

    CAS  Google Scholar 

  63. Wang Z, Zhang S, Murtazina NR, Eremin SA, Shen J (2008) Int J Food Sci Technol 43:114–122

    Article  CAS  Google Scholar 

  64. Zhang S, Wang Z, Nesterenko IS, Eremin SA, Shen J (2007) Int J Food Sci Technol 42:36–44

    CAS  Google Scholar 

  65. Myrtazina NR, Eremin SA, Mozoleva OV, Everest SJ, Brown AJ, Jackman R (2004) Int J Food Sci Technol 39:879–891

    Google Scholar 

  66. Eremin SA, Murtazina NR, Ermolenko DN, Zherdev AV, Mart’ianov AA, Yazynina EV, Michura IV, Formanovsky AA, Dzantiev BB (2005) Anal Lett 38:951–969

    CAS  Google Scholar 

  67. Wang Z, Zhang S, Nesterenko IS, Eremin SA, Shen J (2007) J Agric Food Chem 55:6871–6878

    CAS  Google Scholar 

  68. Colbert DL, Smith DS, Landon J, Sidki AM (1984) Clin Chem 30:1765–1769

    CAS  Google Scholar 

  69. Colbert DL, Childerstone M (1987) Clin Chem 33:1921–1923

    CAS  Google Scholar 

  70. Mel’nichenko OA, Eremin SA, Egorov AM (1996) Zh Anal Khim 51:557–560 (in Russian) [Russ J Anal Chem (Engl Transl) 51:512–515]

    Google Scholar 

  71. Choi MJ, Lee JR, Eremin SA (2002) Food Agric Immunol 14:107–120

    CAS  Google Scholar 

  72. Hong JY, Choi MJ (2002) Biol Pharm Bull 25:1258–1262

    CAS  Google Scholar 

  73. Gaikwad A, Gómez-Hens A, Pérez-Bendito D (1993) Anal Chim Acta 280:129–135

    CAS  Google Scholar 

  74. Perez-Bendito D, Gomez-Hens A, Gaikwad A (1994) Clin Chem 40:1489–1493

    CAS  Google Scholar 

  75. Eremin SA, Matveeva EG, Gomez-Hens A, Perez-Bendito D (1998) Int J Environ Anal Chem 71:137–146

    CAS  Google Scholar 

  76. Sendra B, Panadero S, Eremin S, Gomez-Hens A (1998) Talanta 47:153–160

    CAS  Google Scholar 

  77. Gomez-Hens A, Aguilar-Caballos MP (2003) Comb Chem High Throughput Screening 6:177–182

    CAS  Google Scholar 

  78. Matveeva EG, Popova VA, Eremin SA (1997) J Fluorescence 7:251–256

    CAS  Google Scholar 

  79. Matveeva EG, Samsonova ZV, Eremin SA (1996) Bioorganicheskaya Khimiya 22:931–937 (in Russian) [Russ J Biorg Chem (Engl Transl) 22:815–820]

    Google Scholar 

  80. Adamczyk M, Fishpaugh JR, Heuser KJ, Ramp JM, Reddy RE, Wong M (1998) Tetrahedron 54:3093–3112

    CAS  Google Scholar 

  81. Johnson DK, Combs SM, Parsen JD, Jolley ME (2002) Environ Sci Technol 36:1042–1047

    CAS  Google Scholar 

  82. Johnson DK (1999) Anal Chim Acta 399:161–172

    CAS  Google Scholar 

  83. Gomez-Hens A, Aguilar-Caballos MP (2004) Trends Anal Chem 23:127–136

    CAS  Google Scholar 

  84. Guo XQ, Castellano FN, Li L, Lakowicz JR (1998) Anal Chem 70:632–637

    CAS  Google Scholar 

  85. Terpetschnig E, Szmacinski H, Lakowicz JR (1996) Anal Biochem 240:54–59

    CAS  Google Scholar 

  86. Dürkop A, Lehmann F, Wolfbeis OS (2002) Anal Bioanal Chem 372:688–694

    Google Scholar 

  87. Terpetsching E, Szmacinski H, Lakowicz JR (1995) Anal Biochem 227:140–147

    Google Scholar 

  88. Sanchez-Martınez ML, Aguilar-Caballos MP, Eremin SA, Gomez-Hens A (2005) Anal Chim Acta 553:93–98

    Google Scholar 

  89. Sanchez-Martinez ML, Aguilar-Caballos MP, Eremin SA, Gomez-Hens A (2007) Talanta 72:243–248

    CAS  Google Scholar 

  90. Sanchez Martinez ML, Aguilar Caballos MP, Gomez Hens A (2007) Anal Chem 79:7424–7430

    CAS  Google Scholar 

  91. Ansell RJ (2004) J Chromatogr B 804:151–165

    CAS  Google Scholar 

  92. Hunt CE, Pasetto P, Ansell RJ, Haupt K (2006) Chem Commun (Camb) 16:1754–1756

    Google Scholar 

  93. Hunt CE, Ansell RJ (2006) Analyst 131:678–683

    CAS  Google Scholar 

  94. Ye LW, Le XC, Xing JZ et al (1998) J Chromatogr B 714:59–67

    CAS  Google Scholar 

  95. Wan QH, Le XC (1999) J Chromatogr B 734:31–38

    CAS  Google Scholar 

  96. Lam MT, Le XC (2002) Analyst 127:1633–1637

    CAS  Google Scholar 

  97. Wan QH, Le XC (1999) J Chromatogr A 853:555–562

    CAS  Google Scholar 

  98. Wan QH, Le XC (2000) Anal Chem 72:5583–5589

    CAS  Google Scholar 

  99. Fu H, Guthrie JW, Le XC (2006) Electrophoresis 27:433–441

    CAS  Google Scholar 

  100. Khoschsorur GA, Fruehwirth F, Zelzer S, Stettin M, Halwachs Baumann G (2007) Clin Chim Acta 380:217–221

    Google Scholar 

  101. Graves TL, Zhang Y, Scott JE (2008) Anal Biochem 373:296–306

    CAS  Google Scholar 

  102. Sorme P, Kahl Knutsson B, Wellmar U, Nilsson UJ, Leffler H (2003) Methods Enzymol 362:504–512

    Google Scholar 

  103. Sorme P, Kahl Knutsson B, Huflejt M, Nilsson UJ, Leffler H (2004) Anal Biochem 334:36–47

    Google Scholar 

  104. Aplander K, Tejler J, Toftered J, Carlsson S, Kahl-Knutsson B, Sundin A, Leffler H, Nilsson UJ (2006) Carbohydr Res 341:1363–1369

    CAS  Google Scholar 

  105. Lokesh GL, Rachamallu A, Kishore Kumar GD, Natarajan A (2006) Anal Biochem 352:135–141

    CAS  Google Scholar 

  106. Liu Y, Jiang J, Richardson PL, Reddy RD, Johnson DD, Kati WM (2006) Anal Biochem 356:100–107

    CAS  Google Scholar 

  107. Zhang R, Mayhood T, Lipari P, Wang Y, Durkin J, Syto R, Gesell J, McNemar C, Windsor W (2004) Anal Biochem 331:138–146

    CAS  Google Scholar 

  108. Kenny CH, Ding W, Kelleher K, Benard S, Dushin EG, Sutherland AG, Mosyak L, Kriz R, Ellestad G (2003) Anal Biochem 323:224–233

    CAS  Google Scholar 

  109. Do EU, Choi G, Shin J, Jung W, Kim S (2004) Anal Biochem 330:156–163

    CAS  Google Scholar 

  110. Zhao G, Meier TI, Kahl SD, Gee KR, Blaszczak LC (1999) Antimicrob Agents Chemother 43:1124–1128

    CAS  Google Scholar 

  111. Luk KC, Hyde EG, Trojanowski JQ, Lee VMY (2007) Biochemistry 46:12522–12529

    CAS  Google Scholar 

  112. Nakayama GR, Bingham P, Tan D, Maegley KA (2006) Anal Biochem 351:260–265

    CAS  Google Scholar 

  113. Jolley ME, Nasir MS, Surujballi OP, Romanowska A, Renteria TB, De la Mora A, Lim A, Bolin SR, Michel AL, Kostovic M, Corrigan EC (2007) Vet Microbiol 120:113–121

    CAS  Google Scholar 

  114. Jolley ME, Nasir MS (2003) Comb Chem High Throughput Screening 6:235–244

    CAS  Google Scholar 

  115. Popelka SR, Miller DM, Holen JT, Kelso DM (1981) Clin Chem 27:1198–1201

    CAS  Google Scholar 

  116. Eremin SA, Landon J, Smith DS, Jackman R (1994) Analyst 119:2723–2726

    CAS  Google Scholar 

  117. Eremin SA, Zaitchev SV, Egorov AM, Ermakov AN, Smirnov AV, Izotov BN (1989) Anal Chim Acta 227:287–290

    CAS  Google Scholar 

  118. Kowski TJ, Wu JJ (2000) Comb Chem High Throughput Screening 3:437–444

    CAS  Google Scholar 

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Acknowledgements

We acknowledge the support of the Russia-Flandria project 05-03-34828 “Strategy for express detection of mycotoxins in food products” and NATO grant CBP.NR.CLG.982651 “New strategy for biodetection of explosive nitro-chemicals in environmental samples”.

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  1. MicroPharm Ltd, 51-53 Bartholomew Close, St. Bartholomew’s Hospital, London, EC1A 7BE, UK

    David S. Smith

  2. Division of Chemical Enzymology, Faculty of Chemistry, M.V. Lomonosov Moscow State University, Leninskie Gory, 1, 119992, Moscow, Russia

    Sergei A. Eremin

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Smith, D.S., Eremin, S.A. Fluorescence polarization immunoassays and related methods for simple, high-throughput screening of small molecules. Anal Bioanal Chem 391, 1499–1507 (2008). https://doi.org/10.1007/s00216-008-1897-z

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