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Protective effects of calcitonin gene-related peptide-mediated evodiamine on guinea-pig cardiac anaphylaxis

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Abstract

Previous investigations have indicated that the pharmacological effects of evodiamine, a major alkaloidal component of the dried, unripe fruit of Evodia rutaecarpa Bentham (Rutaceae), are associated with stimulation of calcitonin gene-related peptide (CGRP) release and CGRP prevents cardiac anaphylactic injury. In the present study, the protective effects of evodiamine on cardiac anaphylaxis were examined. Presensitized guinea-pig hearts challenged with specific antigen (bovine serum albumin) caused a marked decrease in coronary flow, left ventricular pressure and its derivatives (±dp/dtmax), an increase in heart rate, and prolongation of P-R interval. Evodiamine (0.3 μM or 1 μM) markedly increased the content of CGRP in the coronary effluent concomitantly with a significant improvement of cardiac function and alleviation of the extension of P-R interval. Evodiamine at the concentration of 1 μM also inhibited the sinus tachycardia. The protective effect of evodiamine on cardiac anaphylaxis was abolished by CGRP8–37, the selective CGRP receptor antagonist. These results suggest that evodiamine possesses a protective effect of cardiac anaphylactic injury and that the effect of evodiamine is related to stimulation of CGRP release.

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Acknowledgements

This study was supported by grants from the Natural Science Foundation No.30200361 and the Ministry of Education, P.R. China.

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Authors and Affiliations

  1. Department of Pharmacology, School of Pharmaceutical Sciences, Central South University, Xiang-Ya Road 88, 410078, Changsha, Hunan, P.R. China

    Wei-Qing Rang, Yan-Hua Du, Chang-Ping Hu, Feng Ye, Gui-Shan Tan, Han-Wu Deng & Yuan-Jian Li

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  1. Wei-Qing Rang
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  2. Yan-Hua Du
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  3. Chang-Ping Hu
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  4. Feng Ye
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  7. Yuan-Jian Li
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Correspondence to Yuan-Jian Li.

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Rang, WQ., Du, YH., Hu, CP. et al. Protective effects of calcitonin gene-related peptide-mediated evodiamine on guinea-pig cardiac anaphylaxis. Naunyn-Schmiedeberg's Arch Pharmacol 367, 306–311 (2003). https://doi.org/10.1007/s00210-002-0677-0

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