Abstract
Objective
The pathogenesis of multiple organ dysfunction syndrome (MODS) in septic shock is mainly caused by maldistribution of tissue perfusion and the amplification of inflammatory responses, which may be modulated by β2-adrenoceptor agonists. We evaluated and compared effects of terbutaline on MODS in a cecal ligation and puncture (CLP) model of sepsis.
Design and setting
Prospective controlled animal study in a university laboratory.
Methods
Male adult Wistar rats received CLP or sham operation followed by the administration of saline or terbutaline (0.3 mg/kg i.v. at 3 and 9 h after CLP).
Measurements and results
At 0, 9 and 18 h after CLP, the changes of hemodynamics, organ function indexes, as well as the plasma interleukin-1β (IL-1β) and nitrite/nitrate levels were examined. At 18 h after CLP, animals were killed and their lungs, livers and kidneys were immediately excised to analyze superoxide anion (O2 −) levels and inducible nitric oxide synthase (iNOS) expression. These organs were also evaluated by pathological study. Terbutaline significantly (1) prevented delayed hypotension and reduced hepatic and renal dysfunction, (2) diminished plasma IL-1β and nitrite/nitrate, lung iNOS expression, tissue O2 − level and the infiltration of neutrophils in the lung and the liver, and (3) improved the 18-h survival rate.
Conclusions
Terbutaline may be developed as an alternative treatment for severe sepsis-induced MODS. The protective effect of terbutaline seems to be through inhibition of proinflammatory mediators and attenuation of oxidant production.
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Acknowledgments
This work was supported by grants NSC 94-2320-B-016-037, 95-2320-B-016-008 and 96-2320-B-016-002 from the National Science Council and DOD 95-07-02, 96-01-08 and 97-08-01 from the National Defense Ministry, Taiwan, ROC
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C.-M. Tsao and S.-J. Chen have made equal contribution to this study.
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Tsao, CM., Chen, SJ., Shih, MC. et al. Effects of terbutaline on circulatory failure and organ dysfunction induced by peritonitis in rats. Intensive Care Med 36, 1571–1578 (2010). https://doi.org/10.1007/s00134-010-1839-z
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DOI: https://doi.org/10.1007/s00134-010-1839-z