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Alleviation Effect of Alginate-Derived Oligosaccharides on Vicia faba Root Tip Cells Damaged by Cadmium

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Abstract

Cadmium has been shown to prevent Vicia faba growth by inhibiting cell mitosis. In this study we investigated the role of Alginate-derived Oligosaccharides (ADO) in alleviating Vicia faba root tip cells damaged by 6 and 8 mg L−1 CdCl2. Micronucleus assay and chromosomal aberration assay were used to determine mitotic index, micronucleus frequency and chromosomal aberration frequency. The results showed that micronucleus frequency of Vicia faba root tip cells was inhibited under all the ADO concentrations. Especially, the inhibition ratio of 0.125% ADO highly reached 66.11 and 67.17% in 6 and 8 mg L−1 CdCl2, respectively. Furthermore, the mitotic index increased (p < 0.05) and chromosomal aberration frequency decreased (p < 0.05) under all the ADO concentrations. This indicated that ADO had a significant alleviation effect on Vicia faba root tip cells damaged by cadmium.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (30870205) and the Liaoning province Natural Science Foundation (20052053).

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  1. College of Chemistry and Life Science, Shenyang Normal University, 253, Huanghe Street, 110034, Shenyang, China

    L. J. Ma, Y. Zhang, N. Bu & S. H. Wang

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  1. L. J. Ma
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  2. Y. Zhang
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  3. N. Bu
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  4. S. H. Wang
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Correspondence to N. Bu.

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Ma, L.J., Zhang, Y., Bu, N. et al. Alleviation Effect of Alginate-Derived Oligosaccharides on Vicia faba Root Tip Cells Damaged by Cadmium. Bull Environ Contam Toxicol 84, 161–164 (2010). https://doi.org/10.1007/s00128-009-9914-2

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  • DOI: https://doi.org/10.1007/s00128-009-9914-2

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