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Development of IRAP- and REMAP-derived SCAR markers for marker-assisted selection of the stripe rust resistance gene Yr15 derived from wild emmer wheat

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Abstract

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Yr15 provides broad resistance to stripe rust, an important wheat disease. REMAP- and IRAP-derived co-dominant SCAR markers were developed and localize Yr15 to a 1.2 cM interval. They are reliable across many cultivars.

Abstract

Stripe rust [Pucinia striiformis f.sp. tritici (Pst)] is one of the most important fungal diseases of wheat, found on all continents and in over 60 countries. Wild emmer wheat (Triticum dicoccoides), which is the tetraploid progenitor of durum wheat, is a valuable source of novel stripe rust resistance genes for wheat breeding. T. dicoccoides accession G25 carries Yr15 on chromosome 1BS. Yr15 confers resistance to virtually all tested Pst isolates; it is effective in durum and bread wheat introgressions and their derivatives. Retrotransposons generate polymorphic insertions, which can be scored as Mendelian markers using techniques such as REMAP and IRAP. Six REMAP- and IRAP-derived SCAR markers were mapped using 1,256 F2 plants derived from crosses of the susceptible T. durum accession D447 (DW1) with its resistant BC3F9 and BC3F10 (B9 and B10) near isogenic lines, which carried Yr15 introgressed from G25. The nearest markers segregated 0.1 cM proximally and 1.1 cM distally to Yr15. These markers were also mapped and validated at the same position in another 500 independent F2 plants derived from crosses of B9 and B10 with the susceptible cultivar Langdon (LDN). SC2700 and SC790, defining Yr15 on an interval of 1.2 cM, were found to be reliable and robust co-dominant markers in a wide range of wheat lines and cultivars with and without Yr15. These markers are useful tags in marker-assisted wheat breeding programs that aim to incorporate Yr15 into elite wheat lines and cultivars for durable and broad-spectrum resistance to stripe rust.

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Acknowledgments

This work was supported by the European union’s sixth framework programme (FP6) in the BioExploit project (No. CT-2005-513949). We thank Dr. T. Fahima for his support of, and multiple contributions to, this project. A.-M. Narvanto and U. Lönnqvist are deeply appreciated for technical assistance. For providing plant materials and pedigrees, the authors acknowledge Drs. A. Grama, Z. Gerechter-Amitai, J. Dubcovsky, R. McIntosh, T. The, M.S. Hovmøller. F. Borum, J. Barrett, and B. Schuiling. We thank Dr. J. Manisterski for providing urediniospores of Puccinia striiformis race 38E134 isolate 5006.

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The authors declare that they have no conflict of interest.

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The experiments comply with the current laws of the countries in which they were performed.

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Authors and Affiliations

  1. Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Urmia University, Urmia, Iran

    Babak Abdollahi Mandoulakani

  2. MTT/BI Plant Genomics Laboratory, Institute of Biotechnology, University of Helsinki, Viikki Biocenter, P.O. Box 65, 00014, Helsinki, Finland

    Elitsur Yaniv, Ruslan Kalendar, Dina Raats & Alan H. Schulman

  3. The University of Sydney Plant Breeding Institute-Cobbitty, PMB 4011, Narellan, NSW, 2567, Australia

    Harbans S. Bariana

  4. Department of Agronomy and Plant Breeding, Faculty of Agricultural Science and Engineering, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

    Mohammad Reza Bihamta

  5. Biotechnology and Food Research, MTT Agrifood Science Finland, Myllytie 1, 31600, Jokioinen, Finland

    Alan H. Schulman

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  1. Babak Abdollahi Mandoulakani
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  2. Elitsur Yaniv
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Correspondence to Alan H. Schulman.

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Communicated by Mingliang Xu.

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Abdollahi Mandoulakani, B., Yaniv, E., Kalendar, R. et al. Development of IRAP- and REMAP-derived SCAR markers for marker-assisted selection of the stripe rust resistance gene Yr15 derived from wild emmer wheat. Theor Appl Genet 128, 211–219 (2015). https://doi.org/10.1007/s00122-014-2422-8

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