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Genetic analysis and detection of the gene MlLX99 on chromosome 2BL conferring resistance to powdery mildew in the wheat cultivar Liangxing 99

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The effectiveness of wheat cultivar Liangxing 99 against powdery mildew was shown to be controlled by a single dominant gene located on a new locus of chromosome 2BL in the bin 2BL2-0.35-0.50.

Abstract

Liangxing 99, one of the most widely grown commercial cultivars in the winter wheat (Triticum aestivum) producing regions in northern China, was shown to provide a broad spectrum of resistance to Blumeria graminis f. sp. tritici (Bgt) isolates originating from that region. Using an F2 population and F2:3 lines derived from a cross of Liangxing 99 × Zhongzuo 9504, genetic analysis demonstrated that a single dominant gene, designated MlLX99, was responsible for the resistance of Liangxing 99 to Bgt isolate E09. The results of molecular analysis indicated that this gene is located on chromosome 2BL and flanked by the SSR marker Xgwm120 and EST-STS marker BE604758 at genetic distances of 2.9 and 5.5 cM, respectively. Since the flanking markers of MlLX99 were previously mapped to the bin 2BL2-0.36-0.50, MlLX99 must be located in this chromosomal region. MlLX99 showed a different resistance reaction pattern to 60 Bgt isolates from Pm6, Pm33, and PmJM22, which were all previously mapped on chromosome 2BL, but differed in their positions from MlLX99. Due to its unique position on chromosome 2BL, MlLX99 appears to be a new locus for resistance to powdery mildew. Liangxing 99 has shown superior yield performance and wide adaptation to different agricultural conditions, which has resulted in its extensive use as a wheat cultivar in China. The identification of resistance gene MlLX99 facilitates the use of this cultivar in the protection of wheat from damage caused by powdery mildew.

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Acknowledgments

We are grateful to Dr. RL Conner of the Morden Research Station, Agriculture and Agri-Food Canada, for critical review of this manuscript. The authors thank Dr. Yilin Zhou of Institute of Plant Protection, Chinese Academy of Agricultural Sciences, and Dr. Hongxing Xu of Center for Agro-Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences for their providing of some Bgt isolates. This study was financially supported by the National Program on Key Basic Research Project (973 Program, 2009CB118304) and Modern Agro-industry Technology Research System (CARS-3-1).

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Authors and Affiliations

  1. The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    Zihui Zhao, Huigai Sun, Wei Song, Longfei Wu, Xiaoming Wang & Hongjie Li

  2. College of Life Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao, 066004, China

    Zihui Zhao, Huigai Sun, Wei Song & Ming Lu

  3. College of Biotechnology, Guilin Medical University, Guilin, 541004, China

    Jiang Huang

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  1. Zihui Zhao
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  2. Huigai Sun
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  3. Wei Song
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  4. Ming Lu
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  5. Jiang Huang
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  7. Xiaoming Wang
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  8. Hongjie Li
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Correspondence to Hongjie Li.

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Communicated by A. E. Melchinger.

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Zhao, Z., Sun, H., Song, W. et al. Genetic analysis and detection of the gene MlLX99 on chromosome 2BL conferring resistance to powdery mildew in the wheat cultivar Liangxing 99. Theor Appl Genet 126, 3081–3089 (2013). https://doi.org/10.1007/s00122-013-2194-6

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