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Identification of quantitative trait loci in rye introgression lines carrying multiple donor chromosome segments

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Abstract

Introgression libraries can be used to make favorable genetic variation of exotic donor genotypes available in the genetic background of elite breeding material. Our objective was to employ a combination of the Dunnett test and a linear model analysis to identify favorable donor alleles in introgression lines (ILs) that carry long or multiple donor chromosome segments (DCS). We reanalyzed a dataset of two rye introgression libraries that consisted of ILs carrying on average about four donor segments. After identifying ILs that had a significantly better per se or testcross performance than the recipient line with the Dunnett test, the linear model analysis was in most instances able to clearly identify the donor regions that were responsible for the superior performance. The precise localization of the favorable DCS allowed a detailed analysis of pleiotropic effects and the study of the consistency of effects for per se and testcross performance. We conclude that in many cases the linear model analysis allows the assignment of donor effects to individual DCS even for ILs with long or multiple donor segments. This may considerably increase the efficiency of producing sub-ILs, because only such segments need to be isolated that are known to have a significant effect on the phenotype.

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Acknowledgments

This article is dedicated to Professor Dr. Dr. h.c. Wolfgang Friedt on the occasion of his 65th birthday. Funding from the German Federal Ministry of Education and Research (BMBF Grants #0312289B and 315951C), the German Federal Ministry of Economics (Aif Grant #KF0141101MD5), the German Federal Minsitry of Food, Agriculture, and Consumer’s Protection (BMELV) via the Federal Agency for Agriculture and Food (BLE) and the “Gemeinschaft zur Förderung der privaten deutschen Pflanzenzüchtung” (GFP), Grant no. PGI-06.01-28-1-43.017-07, and the breeding companies Hybro GmbH & Co. KG, Schenkendorf, and KWS LOCHOW GmbH, Bergen are gratefully acknowledged. We thank M. Raith (University of Hohenheim), J.-C. Gudehus (KWS LOCHOW GmbH, Bergen), Dr. F.J. Fromme (Hybro GmbH & Co KG, Schenkenberg) for conducting the field trials and Dr. V. Korzun (KWS LOCHOW GmbH), Dr. B. Hackauf (JKI, Gross Lüsewitz), and Dr. J. Schondelmaier (SAATEN-UNION Resistenzlabor, Leopoldshöhe) for their support with the marker analyses.

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Authors and Affiliations

  1. Institute of Agronomy and Plant Breeding II, Justus Liebig University, 35392, Giessen, Germany

    Gregory S. Mahone & Matthias Frisch

  2. State Plant Breeding Institute, Universitaet Hohenheim, 70593, Stuttgart, Germany

    Thomas Miedaner

  3. KWS LOCHOW GmbH, 29303, Bergen, Germany

    Peer Wilde

  4. Hybro GmbH & Co KG, 17291, Schenkenberg, Germany

    Heinrich Wortmann

  5. Institute for Evolution and Biodiversity, University of Münster, 48149, Munster, Germany

    K. Christin Falke

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  1. Gregory S. Mahone
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  2. Matthias Frisch
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  3. Thomas Miedaner
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  4. Peer Wilde
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  5. Heinrich Wortmann
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  6. K. Christin Falke
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Corresponding author

Correspondence to Matthias Frisch.

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Communicated by X. Xia.

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Mahone, G.S., Frisch, M., Miedaner, T. et al. Identification of quantitative trait loci in rye introgression lines carrying multiple donor chromosome segments. Theor Appl Genet 126, 49–58 (2013). https://doi.org/10.1007/s00122-012-1958-8

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  • DOI: https://doi.org/10.1007/s00122-012-1958-8

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