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Identification and mapping of two powdery mildew resistance genes in Triticum boeoticum L.

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Abstract

Powdery mildew (PM) caused by Blumeria graminis f. sp. tritici (Bgt), is one of the important foliar diseases of wheat that can cause serious yield losses. Breeding for cultivars with diverse resources of resistance is the most promising approach for combating this disease. The diploid A genome progenitor species of wheat are an important resource for new variability for disease resistance genes. An accession of Triticum boeoticum (AbAb) showed resistance against a number of Bgt isolates, when tested using detached leaf segments. Inheritance studies in a recombinant inbred line population (RIL), developed from crosses of PM resistant T. boeoticum acc. pau5088 with a PM susceptible T. monococcum acc. pau14087, indicated the presence of two powdery mildew resistance genes in T. boeoticum acc. pau5088. Analysis of powdery mildew infection and molecular marker data of the RIL population revealed that both powdery mildew resistance genes are located on the long arm of chromosome 7A. Mapping was conducted using an integrated linkage map of 7A consisting of SSR, RFLP, STS, and DArT markers. These powdery mildew resistance genes are tentatively designated as PmTb7A.1 and PmTb7A.2. The PmTb7A.2 is closely linked to STS markers MAG2185 and MAG1759 derived from RFLP probes which are linked to powdery mildew resistance gene Pm1. This indicated that PmTb7A.2 might be allelic to Pm1. The PmTb7A.1, flanked by a DArT marker wPt4553 and an SSR marker Xcfa2019 in a 4.3 cM interval, maps proximal to PmT7A.2. PmTb7A.1 is putatively a new powdery mildew resistance gene. The powdery mildew resistance genes from T. boeoticum are currently being transferred to cultivated wheat background through marker-assisted backcrossing, using T. durum as bridging species.

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Acknowledgments

This work was carried out under Indo-Swiss collaboration in Biotechnology. The financial support provided by the Department of Biotechnology, Ministry of Science and Technology, Government of India and Swiss Agency for Development and Cooperation (to KS and BK) and the Swiss National Science Foundation (to BK) is gratefully acknowledged. The powdery mildew isolates used in the study were multiplied by Gabriele Büsing. Help received by the first author from Ms Gabriele Büsing, Mr. Gerhard Herren, Dr. Susanne Brunner and Dr. Thomas Wicker University of Zurich is gratefully acknowledged.

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Author notes

  1. Harcharan S. Dhaliwal

    Present address: Akal School of Biotechnology, Eternal University, Baru Sahib, Himachal Pradesh, 173101, India

Authors and Affiliations

  1. School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, 141004, India

    Parveen Chhuneja, Krishan Kumar, Harcharan S. Dhaliwal & Kuldeep Singh

  2. Institute of Plant Biology, University of Zurich, Zurich, Switzerland

    Daniel Stirnweis, Severine Hurni & Beat Keller

Authors
  1. Parveen Chhuneja
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  2. Krishan Kumar
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  3. Daniel Stirnweis
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  4. Severine Hurni
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  5. Beat Keller
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  6. Harcharan S. Dhaliwal
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  7. Kuldeep Singh
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Correspondence to Kuldeep Singh.

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Communicated by P. Langridge.

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Chhuneja, P., Kumar, K., Stirnweis, D. et al. Identification and mapping of two powdery mildew resistance genes in Triticum boeoticum L.. Theor Appl Genet 124, 1051–1058 (2012). https://doi.org/10.1007/s00122-011-1768-4

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