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Iso-lines and inbred-lines confirmed loci that underlie resistance from cultivar ‘Hartwig’ to three soybean cyst nematode populations

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Abstract

Soybean [Glycine max (L.) Merr.] cultivars varied in their resistance to different populations of the soybean cyst nematode (SCN), Heterodera glycines, called HG Types. The rhg1 locus on linkage group G was necessary for resistance to all HG types. However, the loci for resistance to H. glycines HG Type 1.3- (race 14) and HG Type 1.2.5- (race 2) of the soybean cyst nematode have varied in their reported locations. The aims were to compare the inheritance of resistance to three nematode HG Types in a population segregating for resistance to SCN and to identify the underlying quantitative trait loci (QTL). ‘Hartwig’, a soybean cultivar resistant to most SCN HG Types, was crossed with the susceptible cultivar ‘Flyer’. A total of 92 F5-derived recombinant inbred lines (RILs; or inbred lines) and 144 molecular markers were used for map development. The rhg1 associated QTL found in earlier studies were confirmed and shown to underlie resistance to all three HG Types in RILs (Satt309; HG Type 0, P = 0.0001 R 2 = 22%; Satt275; HG Type 1.3, P = 0.001, R 2 = 14%) and near isogeneic lines (NILs; or iso-lines; Satt309; HG Type 1.2.5-, P = 0.001 R 2 = 24%). A new QTL underlying resistance to HG Type 1.2.5- was detected on LG D2 (Satt574; P = 0.001, R 2 = 11%) among 14 RILs resistant to the other HG types. The locus was confirmed in a small NIL population consisting of 60 plants of ten genotypes (P = 0.04). This QTL (cqSCN-005) is located in an interval previously associated with resistance to both SDS leaf scorch from ‘Pyramid’ and ‘Ripley’ (cqSDS-001) and SCN HG Type 1.3- from Hartwig and Pyramid. The QTL detected will allow marker assisted selection for multigenic resistance to complex nematode populations in combination with sudden death syndrome resistance (SDS) and other agronomic traits.

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Acknowledgments

This research was funded over the past 11 years in part by grants from the NSF 9872635, ISA 95-122-04; 98-122-02 and 02-127-03 and USB 2228-6228. The integrated genetic and physical map was based upon work supported by the National Science Foundation under Grant No. 9872635. Any opinions, findings, and conclusions or recommendations expressed in this material were those of the author(s) and do not necessarily reflect the views of the National Science Foundation or United States Department of Agriculture. The continued support of SIUC, College of Agriculture and Office of the Vice Chancellor for Research to SK, JA and DAL was appreciated. The authors thank Drs. P. Gibson, O. Myers Jr. and M. Schmidt for assistance with germplasm development and maintenance from 1991 to 2000. We thank the “Soybean Genome Project”, at DoE Joint Genome Institute, for the timely release of the WGS reads, scaffolds and genome sequence.

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Author notes

  1. J. Shultz

    Present address: School of Biological Sciences, Louisiana Tech University, Ruston, LA, USA

  2. Prakash R. Arelli

    Present address: Soybean Genetics Laboratory, USDA, ARS-MSA, Jackson, TN, 38301, USA

Authors and Affiliations

  1. Plant Biotechnology and Genomics Core-Facility, Department of Plant, Soil, and Agricultural Systems, Southern Illinois University, Carbondale, IL, 62901, USA

    Samreen Kazi, J. Shultz, J. Afzal, Rizwan Hashmi, Mohammed Jasim, Jason Bond & David A. Lightfoot

  2. Center for Excellence in Soybean Research, Teaching and Outreach, Southern Illinois University, Carbondale, IL, 62901, USA

    Samreen Kazi, J. Shultz, J. Afzal & David A. Lightfoot

  3. Department of Plant Biology, Southern Illinois University, Carbondale, IL, 62901, USA

    Rizwan Hashmi

  4. Department of Biotechnology, Al-Anbar Uni, Al-Anbar, Iraq

    Mohammed Jasim

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  1. Samreen Kazi
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Correspondence to David A. Lightfoot.

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Communicated by I. Rajcan.

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Kazi, S., Shultz, J., Afzal, J. et al. Iso-lines and inbred-lines confirmed loci that underlie resistance from cultivar ‘Hartwig’ to three soybean cyst nematode populations. Theor Appl Genet 120, 633–644 (2010). https://doi.org/10.1007/s00122-009-1181-4

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