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The 1-deoxy-d-xylulose 5-phosphate synthase gene co-localizes with a major QTL affecting monoterpene content in grapevine

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Abstract

Muscat flavor is a relevant trait both in winemaking and in fresh grape consumption. From a chemical point of view, it is strongly related to the accumulation of monoterpenes in berries. However, knowledge of the genetic mechanisms underlying its regulation is still limited. The objective of this study was to dissect the genetic determinism of aroma in grapevine by applying the analysis of quantitative trait loci (QTL) and the candidate gene (CG) approach. Two F1 segregating progenies were evaluated through high-resolution gas chromatography–mass spectrometry (HRGC–MS) for the amounts of individual monoterpenes over 3 and 2 years. In the Italia × Big Perlon cross 34 CGs, chosen according to gene ontology (GO) terms, were placed on a complete map and tested for linkage with QTLs for linalool, nerol and geraniol levels. Two CGs mapped within a QTL for linalool content on LG 10. A third one co-localized with a major QTL for the level of the three monoterpenes on LG 5; this gene encodes 1-deoxy-d-xylulose 5-phosphate synthase (DXS), which is the first enzyme in the plastidial pathway of terpene biosynthesis. Depending on these findings, we report the first in silico analysis of grapevine DXS genes based on the whole genome sequence. Further research on the functional significance of these associations might help to understand the genetic control of Muscat flavor.

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Acknowledgments

This research was jointly funded by the EU grant (INCO project MASTER, contract ICA4-2001-10065), the autonomous province of Trento, Italy (QTL-A project) and the Fondazione CARITRO Trento, Italy (Advanced Biology Program). We would like to thank also Girolamo Fanizza and Marco Stefanini for the management, respectively of Pop1 and Pop2, Alessandro Cestaro for his support in bioinformatic analysis, and Silvia Carlin for her contribution in metabolite profiling.

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Correspondence to Laura Costantini.

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Communicated by H. Nybom.

J. Battilana and L. Costantini equally contributed to the work.

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Battilana, J., Costantini, L., Emanuelli, F. et al. The 1-deoxy-d-xylulose 5-phosphate synthase gene co-localizes with a major QTL affecting monoterpene content in grapevine. Theor Appl Genet 118, 653–669 (2009). https://doi.org/10.1007/s00122-008-0927-8

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