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Pyramiding and dissecting disease resistance QTL to barley stripe rust

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Abstract

Quantitative resistance (QR) to disease is usually more durable than qualitative resistance, but its genetic basis is not well understood. We used the barley/barley stripe rust pathosystem as a model for the characterization of the QR phenotype and associated genomic regions. As an intermediate step in the preparation of near-isogenic lines representing individual QTL alleles and combinations of QTL alleles in a homogeneous genetic background, we developed a set of QTL introgression lines in a susceptible background. These intermediate barley near-isogenic (i-BISON) lines represent disease resistance QTL combined in one-, two-, and three-way combinations in a susceptible background. We measured four components of disease resistance on the i-BISON lines: latent period, infection efficiency, lesion size, and pustule density. The greatest differences between the target QTL introgressions and the susceptible controls were for the latter three traits. On average, however, the QTL introgressions also had longer latent periods than the susceptible parent (Baronesse). There were significant differences in the magnitudes of effects of different QTL alleles. The 4H QTL allele had the largest effect, followed by the alleles on 1H and 5H. Pyramiding multiple QTL alleles led to higher levels of resistance in terms of all components of QR except latent period.

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Acknowledgements

This work was supported in part by the USDA-NRI program (Plant–Microbe Interactions) and the United States Barley Genome Project (USBGP). We thank Mary Moore, Tanya Filichkin, Ann Corey, and graduate and undergraduate students in the OSU barley program for their technical support in the lab and greenhouse.

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Authors and Affiliations

  1. Department of Crop and Soil Science, Oregon State University, Corvallis, OR, 97331-3002, USA

    K. L. Richardson, M. I. Vales, J. G. Kling & P. M. Hayes

  2. Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR, 97331-2902, USA

    C. C. Mundt

Authors
  1. K. L. Richardson
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  2. M. I. Vales
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  3. J. G. Kling
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  4. C. C. Mundt
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  5. P. M. Hayes
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Correspondence to P. M. Hayes.

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Communicated by F. Ordon

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Richardson, K.L., Vales, M.I., Kling, J.G. et al. Pyramiding and dissecting disease resistance QTL to barley stripe rust. Theor Appl Genet 113, 485–495 (2006). https://doi.org/10.1007/s00122-006-0314-2

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  • DOI: https://doi.org/10.1007/s00122-006-0314-2

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