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Ty1-copia retrotransposon-based SSAP marker development in cashew (Anacardium occidentale L.)

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Abstract

The most popular retrotransposon-based molecular marker system in use at the present time is the sequence-specific amplification polymorphism (SSAP) system . This system exploits the insertional polymorphism of long terminal repeat (LTR) retrotransposons around the genome. Because the LTR sequence is used to design primers for this method, its successful application requires sequence information from the terminal region of the mobile elements . In this study, two LTR sequences were isolated from the cashew genome and used successfully to develop SSAP marker systems. These were shown to have higher levels of polymorphism than amplified fragment length polymorphic markers for this species.

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Authors and Affiliations

  1. Plant Research Unit, University of Dundee at SCRI, Invergowrie, Dundee, DD2 5DA, UK

    N. H. Syed, S. Sureshsundar & A. J. Flavell

  2. School of Plant Sciences, University of Reading, Reading, Berkshire, RG6 6AS, UK

    M. J. Wilkinson & J. J. V. Cavalcanti

  3. Scottish Crop Research Institute, Dundee, DD2 5DA, UK

    N. H. Syed

  4. Regional Research Laboratory-Jorhat, Jorhat, 785 006, Assam, India

    B. S. Bhau

Authors
  1. N. H. Syed
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  2. S. Sureshsundar
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  3. M. J. Wilkinson
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  4. B. S. Bhau
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  5. J. J. V. Cavalcanti
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  6. A. J. Flavell
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Corresponding author

Correspondence to N. H. Syed.

Additional information

Communicated by E. Guiderdoni

N.H. Syed and S. Sureshsundar contributed equally to this investigation.

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Syed, N.H., Sureshsundar, S., Wilkinson, M.J. et al. Ty1-copia retrotransposon-based SSAP marker development in cashew (Anacardium occidentale L.). Theor Appl Genet 110, 1195–1202 (2005). https://doi.org/10.1007/s00122-005-1948-1

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  • DOI: https://doi.org/10.1007/s00122-005-1948-1

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