Abstract
Bacterial artificial chromosome (BAC) libraries have been widely used in different aspects of genome research. In this paper we report the construction of the first mungbean (Vigna radiata L. Wilczek) BAC libraries. These BAC clones were obtained from two ligations and represent an estimated 3.5 genome equivalents. This correlated well with the screening of nine random single-copy restriction fragment length polymorphism probes, which detected on average three BACs each. These mungbean clones were successfully used in the development of two PCR-based markers linked closely with a major locus conditioning bruchid (Callosobruchus chinesis) resistance. These markers will be invaluable in facilitating the introgression of bruchid resistance into breeding programmes as well as the further characterisation of the resistance locus.
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Acknowledgements
The authors are grateful to Dr. Nevin Young (University of Minnesota, St. Paul, Minn.) for his kind donation of some of the RFLP clones used in this study, and to Drs. Adele Schmidt, Karen Aitken and Lynne McIntyre and two anonymous reviewers for their critical review of the manuscript. This project was partially funded by the Grains Research and Development Corporation (grant no. CSC46). The experiments described in this paper comply with the current laws of Australia, where the experiments were conducted.
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Communicated by F.J. Muehlbauer
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Miyagi, M., Humphry, M., Ma, Z.Y. et al. Construction of bacterial artificial chromosome libraries and their application in developing PCR-based markers closely linked to a major locus conditioning bruchid resistance in mungbean (Vigna radiata L. Wilczek). Theor Appl Genet 110, 151–156 (2004). https://doi.org/10.1007/s00122-004-1821-7
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DOI: https://doi.org/10.1007/s00122-004-1821-7