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Comparative AB-QTL analysis in barley using a single exotic donor of Hordeum vulgare ssp. spontaneum.

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Abstract

This paper reports on the first comparative advanced backcross quantitative trait locus (AB-QTL) study in barley. The BC2F2 population H×101 was generated from crossing var. Harry [H; Hordeum vulgare ssp. vulgare (Hv)] with ISR101-23 [101; H. v. ssp. spontaneum (Hsp)]. The results of the AB-QTL analysis for 13 quantitative traits are presented and, subsequently, compared with the AB-QTL study of the barley cross Apex × ISR101-23 (A×101; Pillen et al., Theor Appl Genet 107:340–352). Both AB populations share the exotic Hsp donor accession ISR101-23. In H×101, 108 putative QTLs (17%) were identified among the 650 marker×trait combinations tested. Altogether 52 (48 %) favorable effects were identified from the exotic parent. At these marker loci, the homozygous Hsp genotype was associated with an improvement in the trait compared to the homozygous Hv genotype. The percentage of QTLs detected in H×101 was comparable to that in A×101 (17% vs. 15%), however more favorable exotic QTL alleles were located in H×101 than in A×101 (48% vs. 34%). In H×101, the Hsp QTL allele at EBmac0679[4H] was associated with a yield increase of 5.9% averaged across the six environments tested. A comparison of putative QTLs between H×101 and A×101 was based on 26 shared SSR markers. In total, 26% of the putative QTLs could be detected simultaneously in both AB populations. This finding indicates that only a portion of the QTL effects of the donor allele can be transferred from one elite recipient to the next.

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Acknowledgements

We wish to thank Dr. Eberhard Laubach and Dr. Volker Lein and their teams for conducting field experiments and collecting trait data at the breeding companies Nordsaat and Ackermann, respectively. We also thank Dr. Lizzy Kuntze and her team for measuring quality traits at the Nordsaat laboratory. In addition, the excellent technical assistance of Karola Müller and the technical team of our experimental station Dikopshof at Wesseling is appreciated. We are indebted to Prof. G. Fischbeck, Technical University, München, for providing the wild barley accession ISR101-23. This work was supported by grants Pi339/1-1 and Pi339/1-2 from the Deutsche Forschungsgemeinschaft (DFG).

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Authors and Affiliations

  1. Department of Crop Science and Plant Breeding, University of Bonn, Katzenburgweg 5, 53115, Bonn, Germany

    K. Pillen & J. Léon

  2. KWS, Kleinwanzlebener Saatzucht AG, Grimsehlstr. 31, 37555, Einbeck, Germany

    A. Zacharias

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  1. K. Pillen
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  2. A. Zacharias
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  3. J. Léon
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Correspondence to K. Pillen.

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Communicated by G. Wenzel

This work is dedicated to the 65th birthday of Prof. Dr. H.H. Geiger, Department of Plant Breeding, Seed Science and Population Genetics, University of Hohenheim, Germany.

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Pillen, K., Zacharias, A. & Léon, J. Comparative AB-QTL analysis in barley using a single exotic donor of Hordeum vulgare ssp. spontaneum.. Theor Appl Genet 108, 1591–1601 (2004). https://doi.org/10.1007/s00122-004-1586-z

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