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Barrier protective activities of curcumin and its derivative

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Abstract

Aim and objective

Curcumin, a poly-phenolic compound, possesses diverse pharmacologic activities. However, the barrier protective functions of curcumin or its derivative have not yet been studied. The objective of this study was to investigate the barrier protective activities of curcumin and its derivative (bisdemethoxycurcumin, BDMC) on lipopolysaccharide (LPS) barrier disruption in human umbilical vein endothelial cells (HUVECs) were investigated.

Methods

The barrier protective effects of curcumin and BDMC such as permeability, expression of cell adhesion molecules, monocytes adhesion and migration toward HUVECs were tested.

Results

Curcumin and BDMC inhibited LPS-induced barrier permeability, monocyte adhesion and migration; inhibitory effects were significantly correlated with inhibitory functions of curcumin and BDMC on LPS-induced cell adhesion molecules (vascular cell adhesion molecules, intracellular cell adhesion molecule, E-selectin). Furthermore, LPS-induced nuclear factor-κB (NF-κB) activation and tumor necrosis factor-α (TNF-α) release from HUVECs were inhibited by curcumin and BDMC. Surprisingly, the barrier protective activities of BDMC were better than those of curcumin, indicating that the methoxy group in curcumin negatively regulated barrier protection function of curcumin.

Conclusion

Given these results, curcumin or its derivative, BDMC, showed barrier protective activities and they could be a therapeutic candidates for various systemic inflammatory diseases.

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Abbreviations

LPS:

Lipopolysaccharide

CAM:

Cell adhesion molecule

VCAM:

Vascular cell adhesion molecules

ICAM:

Intracellular cell adhesion molecule

NF-κB:

Nuclear factor-κB

TNF:

Tumor necrosis factor

BDMC:

Bisdemethoxycurcumin

TEM:

Transendothelial migration

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Acknowledgments

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government [MEST] (No. 2011-003410, 2011-0026695, 2011-0030124).

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Authors and Affiliations

  1. Laboratory of Microvascular Circulation Research, NEUORNEX Inc., Daegu, 711-823, Republic of Korea

    Dong-Chan Kim

  2. Department of Anatomy and Histology, College of Oriental Medicine, Daegu Haany University, Gyeongsan, 712-715, Republic of Korea

    Sae-Kwang Ku

  3. Department of Biochemistry and Cell Biology, School of Medicine, Kyungpook National University, 1370 Sankyuk-dong, Buk-gu, Daegu, 702-701, Republic of Korea

    Wonhwa Lee

  4. College of Pharmacy, Research Institute of Pharmaceutical Sciences, Kyungpook National University, 1370 Sankyuk-dong, Buk-gu, Daegu, 702-701, Republic of Korea

    Wonhwa Lee & Jong-Sup Bae

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  1. Dong-Chan Kim
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Correspondence to Jong-Sup Bae.

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Responsible Editor: Ian Ahnfelt-Rønne.

D.-C. Kim and S.-K. Ku equally contributed to this work.

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Kim, DC., Ku, SK., Lee, W. et al. Barrier protective activities of curcumin and its derivative. Inflamm. Res. 61, 437–444 (2012). https://doi.org/10.1007/s00011-011-0430-6

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  • DOI: https://doi.org/10.1007/s00011-011-0430-6

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