Summary
Monocerin is a benzopyran fungal toxin with broad activity on plants, fungi and insects. Its effect upon cell cycle progression has been analyzed in maize roots. Meristematic cells were synchronized by treatment with aphidicolin. Flow cytometric DNA analysis and mitotic indices indicated durations of 1.5 h, 5 h, 2 h and 1 h for respectively G1, S, G2 and M phases of the normal cell cycle at 25°C. Treatment of these synchronized meristems with 0.5 mM monocerin during release after an aphidicolin block produced a short delay in S phase and then a more important delay (about 2.5 h) in entry into mitosis. Treatments for similar durations (3 h) during progression through the cycle revealed two periods of action of monocerin. The first appears to be mid to late S and the second one G2, before the transition point between G2 and M. Action on either one of these target periods could lead to a delay in the G2/M transition, but these two responses did not appear to be additive.
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Abbreviations
- APH:
-
Aphidicolin
- CV:
-
Coefficient of variation
- DAPI:
-
Diamidinophenylindole
- DMSO:
-
Dimethyl sulfoxide
- EDTA:
-
Ethylenediaminetetraacetic acid
- HPLC:
-
High pressure liquid chromatography
- MI:
-
Mitotic index
- SD:
-
Standard deviation
- UV:
-
ultraviolet light
References
Aldridge DC, Turner WB (1970) J Chem Soc C:2598–2601
Antosiewicz D (1990) Cytobiol.28:79–96
Brown SC, Bergounioux C, Tallet S, Marie D (1991). In: I. Negrutiu and G. Gharti-Chhetri (eds) A laboratory guide for plant cellular and molecular plant biology, Birkhaüser Verlag, Basel, pp326–345.
Claydon N, Grove JF, Pople M (1979) J of Invert Pathol 33:364–367
Cuq F, Herrmann-Gorline S, Klaebe A, Rossignol M, Petitprez M (1993) Phytochemistry 34: 1265–1270
Darzynkiewicz Z, Tragonos F, Melamed MR (1980) Cytometry 1:98–108
Dean PN (1980) Cell Tissue Kinet 13:299–308
Dean PN, Jett JH (1974) J Cell Biol 60:523–529
Essad S, Bousquet JF (1981) Agronomie 1:689–694
Evans LS, Van't Hof J (1975) Exp Cell Res 90:401–410
Galbraith DW, Harkins KR, Maddox JM, Ayres NM, Dharam, PS, Firoozabady E (1983) Science 220:1049–1051
Hosaka H, Takagi M (1992) Plant Physiol 99:1650–1656
Ikegami S, Taquchi T, Ohashi M (1978) Nature 275:458–460
Lehner CF, O'Farrell P (1989) Cell 56:957–968
Lewin B (1990) Cell 61:743–752
Minshull J, Golsteyn R, Hill CS, Hunt T (1990) EMBO J 9:2865–2875
Nurse P (1990) Nature 344:503–508
Pedrali-Noy G, Spadari S, Miller-Faures A, Kruppa J, Koch G (1980) Nucleic Acids Res 8:377–387
Pines J, Hunter T (1989) Cell 58:833–846
Pines J, Hunter T (1991) J Cell Biol 115:1–17
Robeson DJ, Strobel GA (1982) Agric Biol Chem 46:2681–2683
Sala F, Galli G, Pedrali-Noy G, Spadari S (1986) Methods in Enzymology 118:87–96.
Sala F, Galli MG, Pedrali-Noy G, Spadari S (1985). In: L. van Vloten-Doting, G.S.P. Groot and T.C. Hall (eds) Molecular Form and Function of the Plant Genome Plenum Publishing Corporation, New York, pp 141–153
Sala F, Parisi B, Amileni AR, Pedrali-Noy G, Spadari S (1980) Febs Lett 117:93–98
Scott FE, Simpson TJ, Trimble LA, Vederas JC (1984) J. Chem. Soc. Commun:756–758
Sgorbati E, Levi M, Chiatante D (1989) Physiol Plant 75:479–484
Sgorbati S, Levi M, Sparvoli E, Trezzi F, Lucchini G (1986) Physiol Plant 68:471–476
Sgorbati S, Sparvoli E, Levi M, Galli G, Citterio S, Chiatante D (1991) Physiol Plant 81:507–512
Verma RS (1980) Cytologia 45:327–333
Whitfield WGF., Gonzalez C, Sanchez-Herrero E, Glover DM (1989) Nature 338:337–340
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Communicated by A. M. Boudet
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Cuq, F., Brown, S.C., Petitprez, M. et al. Effects of monocerin on cell cycle progression in maize root meristems synchronized with aphidicolin. Plant Cell Reports 15, 138–142 (1995). https://doi.org/10.1007/BF01690271
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DOI: https://doi.org/10.1007/BF01690271