Summary
Temperature controls the developmental fate of isolated Brassica napus microspores in vitro. Culture at 32.5°C leads to sporophytic development and the formation of embryos. Here we show that culture at 17.5°C leads to gametophytic development, and the formation of pollen-like structures at high frequencies (up to 80% after 7 days in culture). Early stages of both developmental pathways are observed in culture at 25.0°C, and embryos are produced at low frequencies (0.7%) at that temperature. Culturing B. napus microspores at 32.5°C versus 17.5°C brings the switch from gametophytic to sporophytic development under simple experimental control and provides a convenient tool for investigating the cellular and molecular mechanisms controlling this developmental switch.
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Communicated by K. Glimelius
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Custers, J.B.M., Cordewener, J.H.G., Nöllen, Y. et al. Temperature controls both gametophytic and sporophytic development in microspore cultures of Brassica napus . Plant Cell Reports 13, 267–271 (1994). https://doi.org/10.1007/BF00233317
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DOI: https://doi.org/10.1007/BF00233317