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Antagonistic effect of 6-pentyl-alpha-pyrone produced by Trichoderma harzianum toward Fusarium moniliforme

Antagonistische Wirkung von 6-Pentyl-alpha-pyron aus Trichoderma harzianum gegen Fusarium moniliforme

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Abstract

The interactions between eight Trichoderma spp. isolates and Fusarium moniliforme were investigated. The results obtained from dual culture and the tests on volatile activity showed that the two isolates of T. harzianum T23 and T16 exhibited considerable antagonistic potential. Quantitative analysis of 6-pentyl-alpha-pyrone (6PAP), purified from culture filtrate of T. harzianum isolate T23, revealed that the optimal harvesting time for 6PAP production was 12 days post inoculation (dpi) (72.3 mg l–1). In in vitro studies, 6PAP demonstrated potent inhibitory properties upon mycelial growth and sporulation of F. moniliforme in both volatile and non-volatile tests as well as on conidia germination and length of germ tubes. Mycelial growth was inhibited by 93.5% using 250 µg ml–1 of 6PAP. Conidia production of the target fungus was inhibited by 250 µg ml–1 of 6PAP in non-volatile and volatile assays by 98.0% and 78.6%, respectively. Germ tube growth was also inhibited by more than 94% using 300 µg ml–1 of 6PAP. The results obtained demonstrated that the metabolite 6PAP exhibited rather fungistatic than fungicidal effects.

Zusammenfassung

Die Interaktionen zwischen acht Trichoderma spp.-Isolaten und Fusarium moniliforme wurden untersucht. Die mit Dualkulturen erzielten Ergebnisse und die Tests zur volatilen Wirkung zeigten, dass zwei T. harzianum-Isolate, T23 und T16, beträchtliches antagonistisches Potential aufwiesen. Quantitative Analyse von 6-Pentyl-alpha-pyron (6PAP), aus dem Kulturfiltrat von T. harzianum-Isolat 23 gereinigt, ergab eine optimale Produktion von 6PAP nach 12-tägiger Inkubation (dpi) (72,3 mg l–1). In in-vitro-Studien zeigten deutliche hemmende Eigenschaften von 6PAP auf Myzelwachstum und Sporulation von F. moniliforme sowohl im nicht volatilen als auch im volatilen Test sowie auf die Konidienkeimung und Keimschlauchlänge. Das Myzelwachstum wurde um 93,5% durch 250 µg ml–1 6PAP gehemmt. Die Konidienproduktion von F. moniliforme wurde durch 250 µg ml–1 6PAP in den nicht volatilen und volatilen Tests um 98,0 bzw. 78,6% gehemmt. Das Keimschlauchwachstum wurde durch 300 µg ml–1 6PAP mehr als 94% inhibiert. Die Ergebnisse zeigten ferner, dass 6PAP eine eher fungistatische als fungizide Wirkung aufwies.

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El-Hasan, A., Walker, F., Schöne, J. et al. Antagonistic effect of 6-pentyl-alpha-pyrone produced by Trichoderma harzianum toward Fusarium moniliforme. J Plant Dis Prot 114, 62–68 (2007). https://doi.org/10.1007/BF03356205

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  • DOI: https://doi.org/10.1007/BF03356205

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