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Greenhouse screening of the saprophytic resident microflora for control of leaf spots of wheat (Triticum aestivum)

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Abstract

Ten microorganisms of the epiphytic microflora of wheat leaves in Buenos Aires Province, Argentina, were evaluated under greenhouse conditions as potential biocontrol agents of the pathogensAlternaria triticimaculans, Bipolaris sorokiniana, Drechslera tritici-repentis andSeptoria tritici in two application sequences (prior to or together with the pathogens). The antagonists significantly reduced the expression of the diseases on wheat plants compared with control plants not inoculated with the antagonists. Maximum percentage of reduction of the necrotic lesion area (NLA) (40–55%) ofS. tritici resulted whenCryptococcus sp.,Rhodotorula rubra andPenicillium lilacinwn were sprayed on leaves prior to inoculations with the pathogen.Bacillus sp.,Cryptococcus sp.,Fusarium moniliforme var.anthophylium,P. lilacinum andR. rubra reduced significantly (34–52%) the NLA ofB. sorokiniana in both of the application sequences. The best antagonistic effect againstA. triticimaculans was shown byAspergillus niger, Bacillus sp.,Chaetomium globosum, F. moniliforme var.anthophylium andNigrospora sphaerica, with a NLA reduction from 21% to 35% in the co-inoculation or in the sequential application. All microorganisms exceptN. sphaerica performed better than the control againstD. tritici-repentis. The area under disease progress curve (AUDPC) of the pathogens appeared to progress similarly, but at lower values, in treated plants than in untreated controls. The two yeasts and the bacteria decreased AUDPC to 50–55% ofS. tritici andB. sorokiniana compared with the control in both application sequences, whereas the maximum efficacy againstA. triticimaculans was reached byN. sphaerica andA. niger for the sequential application and byF. moniliforme var.anthophylium for the co-inoculation. If the parasitism occurs also in nature, application of antagonists for biological control might provide the opportunity to compete with the pathogens and regulate their colonization in wheat leaves.

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References

  1. Alippi, A.M., Perelló, A.E., Sisterna, M.N., Greco, N.M. and Cordo, C. (2000) Potential of spore-forming bacteria as biocontrol agents of wheat foliar diseases under laboratory and greenhouse conditions.Z. Pflanzenkr. Pflanzenschutz 107:155–169.

    Google Scholar 

  2. Andrews, J.H. (1985) Strategies for selecting antagonistic microorganisms from the phylloplane. pp. 31–45.in: Windels, C.E. and Lindow, S.E. [Eds.] Biological Control on the Phylloplane. APS Press, St. Paul, MN, USA.

    Google Scholar 

  3. Andrews J.H. (1992) Biological control in the phyllosphere.Annu. Rev. Phytoputhol. 30:603–635.

    Article  CAS  Google Scholar 

  4. Andrews, J.H. (1992) The screening of microorganisms antagonistic to phytopathogens.Pes. Agropec. Bras. 27:165–174.

    Google Scholar 

  5. Baker, K.F. and Cook, R.J. (1974) Biological Control of Plant Pathogens. W.H. Freeman and Co., San Francisco, CA, USA. pp. 43–64.

    Google Scholar 

  6. Bettiol, W. (1991) Selejao de microrganismos antagonicos a fitopatógenos. pp. 223–236.in: Bettiol, W. [Ed.] Controle Biológico de Ooencas de Plantas. EMBRAPA-CNPDA, JaguarióAna, Brasil.

    Google Scholar 

  7. Blakeman, J.P. and Fokkema, N.J. (1982) Potential for biological control of plant diseases on the phylloplane.Annu. Rev. Phytopathol. 20:167–192.

    Article  Google Scholar 

  8. Cook, R.J. and Baker, K.F. (1983) The Nature and Practice of Biological Control of Plant Pathogens. APS Press, St. Paul, MN, USA. pp. 1–29.

    Google Scholar 

  9. Cook, R.J. and Veseth, J. (1991) Wheat health management. Plant Health Series. APS Press, St. Paul, MN, USA.

    Google Scholar 

  10. Diem, H.G. (1969) Micro-organismes de la surface des feuilles. 3. Effet de la competition nutritive sur la germination des spores d’une souche d’Helminthosporium.Bull. Ec. Natl. Super. Aaron. Nancy 11:18–25.

    Google Scholar 

  11. Dubos, B. and Bulit, J. (1981) Filamentous fungi as biocontrol agents on aerial plant surfaces, pp. 353–356.in: Blakeman, J.P. [Ed.] Microbial Ecology of the Phylloplane. Academic Press, London, UK.

    Google Scholar 

  12. Elad, Y. and Zimand, G. (1992) Integrated biological chemical control of grey mould, pp. 272–276.in: Verhoeff, R., Williamsen, B. and Malathrakis, N.E. [Eds.] Recent Advances inBotrytis Research. Proc. XthBotrytis Symposium. Pudoc Scientific Publishers, Wageningen, the Netherlands.

    Google Scholar 

  13. Fokkema, N.J. (1993) Opportunities and problems of control of foliar pathogens with microorganisms.Pestic. Sci. 37:411–416.

    Article  Google Scholar 

  14. Fokkema, N.J., Den Houter, J.G., Kosterman, Y.J.C. and Nelis, A.L. (1979) Manipulation of yeasts grown on wheat leaves and their antagonistic effect onCochliobolus sativus andSeptoria nodorum.Trans. Br. Mycol. Soc. 72:19–29.

    Article  Google Scholar 

  15. Fokkema, N.J. and Meulen, F. van der (1976) Antagonisms of yeast-like phyllosphere fungi againstSeptoria nodorum on wheat leaves.Neth. J. Plant Pathol. 82:13–16.

    Article  Google Scholar 

  16. Hemming, B.C. (1990) Bacteria as antagonists in biological control of plant pathogens. pp. 223–242.in: Baker, R. and Dunn, P. [Eds.] New Directions in Biological Control. Alan R. Liss, Inc. New York, NY.

    Google Scholar 

  17. Heuvel, J. van der (1970) Antagonistic effects of epiphytic microorganisms on infection of dwarf bean leaves byAlternaria zinniae. Diss., Willie Commelin Scholten Phytopathological Lab., Baarn, the Netherlands.

    Google Scholar 

  18. Jacobsen, B. and Blackman, A. (1993) Biological and cultural plant disease control: alternatives and supplements to chemicals in IPM systems.Plant Dis. 7:311–315.

    Google Scholar 

  19. Kholi, M.M., Annone, J.G. and Garcia, R. [Eds.] (1996) Las enfermedades del trigo en el Cono Sur. Curso de Manejo de Enfermedades del Trigo. Pergamino, Argentina, 1995.

    Google Scholar 

  20. Levy, E., Eyal, Z., Carmely, S., Kashman, Y. and Chet, I. (1989) Suppression ofSeptoria tritici andPuccinia recondita of wheat by an antibiotic-producing fluorescent pseudomonad.Plant Pathol. 38:564–570.

    Article  Google Scholar 

  21. Levy, E., Eyal, Z. and Chet, I. (1992) Inhibition ofSeptoria tritici and other phytopathogenic fungi byPseudomonas fluorescens and its antibiotics.Plant Pathol. 41:335–341.

    Article  CAS  Google Scholar 

  22. Li, B. and Sutton, J.C. (1995) Evaluation of leaf-associated microorganisms for biocontrol of tan spot in wheat foliage.Fitopatol. Bras. 20:545–552.

    Google Scholar 

  23. Luz, W.C. da (1982) Controle biologico das manchas foliares do trigo a traves de microorganismos da filosfera.Fitopatol. Bras. 7:530–531.

    Google Scholar 

  24. Luz, W.C. da (1991) Controle biologico das doencas foliares do trigo. pp. 383–388.in: Bettiol, W. [Ed.] Controle Biologico de Doencas de Plantas. EMBRAPA, Brasil.

    Google Scholar 

  25. McBride, R.P. (1971) Micro-organisms interactions in the phyllosphere of larch. pp. 545–555.in: Preece, T.F. and Dickinson, C.H. [Eds.] Ecology of leaf surface micro-organisms. Academic Press, London, UK.

    Google Scholar 

  26. Newhook, F.J. (1951) Microbial control ofBotrytis cinerea Pers. 1. The role of pH changes and bacterial antagonism.Ann. Appl. Biol. 38:169–184.

    Article  Google Scholar 

  27. Nielsen, P. and Sorensen, J. (1997) Multi-target and medium independent fungal antagonism by hydrolytic enzymes inPaenibacillus polymyxa andBacillus pumilus strains from barley rhizosphere.FEMS Microbiol. Ecol. 22:183–192.

    Article  CAS  Google Scholar 

  28. Perelló, A. (1998) Interacciones entre la microflora saprotrofa y patógenos foliares del filoplano del trigo. Tesis Doctoral. Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, Argentina, pp. 70–117.

    Google Scholar 

  29. Perelló, A., Cordo, C. and Simón, M.R. (1996) A new disease of wheat caused byAlternaria triticimaculans in Argentina.Agronomic 16:107–112.

    Article  Google Scholar 

  30. Perelló, A., Monacó, C. and Cordo, C. (1997) Evaluation ofTrichoderma harzianum andGliodadium ro.ieum in controlling leaf blotch of wheat (Septoria tritici) under in vitro and greenhouse conditions.Z. Pflanzenkr. Pflanzenschutz. 104:588–598.

    Google Scholar 

  31. Perello, A., Simon, M.R., Cordo, C. and Arambarri, A.M. (1998) Use of phylloplane fungi to control leaf blotch of wheat caused bySeptoria tritici. Proc. Sixth Int. Mycol. Congress (Jerusalem, Israel), p. 71.

  32. Perello, A., Simon, M.R., Cordo, C. and Arambarri, A.M. (1998) Microscopic interactions between foliar pathogens and the mycoftora of wheat phylloplane.Proc. Sixth Int. Mycol. Congress (Jerusalem, Israel), p. 72.

  33. Perelló, A., Simón, M.R., Sisterna, M., Cordo, C. and Arambarri, A.M. (1998) Efecto de microorganismos saprotrofos del filoplano del trigo en el control biológico de patógenos foliares.1° Congr. Argentino de Control Biológico de Enfermedades de las Plantas (INTA, UBA) (Buenos Aires, Argentina), p. 22.

  34. Perelló, M.R., Simón, A.M, Sisterna, M., Cordo, C. and Arambarri, A.M. (2001) Microflora of wheat (Triticum aestivum L.) in Buenos Aires Province (Argentina) and its possible significance in biological control of foliar pathogens. Z.Pflanzenkr. Pflanzenschutz. 108(5): in press.

  35. Podile, A. and Prekash, A. (1996) Lysis and biological control ofAspergillus niger byBacillus subtilis.Can. J. Microbiol. 42:533–538.

    Article  PubMed  CAS  Google Scholar 

  36. Porter, C.L. (1924) Concerning the characters of certain fungi as exhibited by the growth in the presence of other fungi.Am. J. Bot. 11:168–188.

    Article  Google Scholar 

  37. Robbs, C. (1991) Bacterias como agentes de controle biologico de fitopatógenos. p. 49.in: Bettiol, W. [Ed.] Controle Biologico da Doencas en Plantas. EMBRAPA, Brasil.

    Google Scholar 

  38. Rodriguez, R. and Bockus, W. (1996) Differences among isolates ofPyrenophora tritici-repentis in production of conidia on wheat leaves.Plant Dis. 80:686–694.

    Google Scholar 

  39. Shanner, G. and Finney, R.E. (1977) The effect of nitrogen fertilization on the expression of slow-mildewing resistance in wheat.Phytopathology 72:154–158.

    Article  Google Scholar 

  40. Shipton, W.A., Boyd, W.R.J., Rosielle, A.A. and Shearer, B.I. (1971) The commonSeptoria diseases of wheat,Septoria nodorum, Septoria tritici.Bot. Rev. 37:231–262.

    Article  Google Scholar 

  41. Simmons, E. (1994)Alternaria themes and variations.Mycotaxon 50:409–427.

    Google Scholar 

  42. Windels, C.E. and Lindow, S.W. [Eds.] (1985) Biological Control on the Phylloplane. APS Press, St. Paul, MN, USA. pp. 6–30.

    Google Scholar 

  43. Zadoks, J.C., Chang, T.T. and Konzak, C.F. (1974) A decimal code for growth stages of cereals.Weed Res. 14:415–421.

    Article  Google Scholar 

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Author information

Authors and Affiliations

  1. Centro de Investigaciones de Fitopatologia, Fac. de Ciencias Agrarias y Forestales, Univ. Nacional de La Plata, Buenos Aires, Argentina

    A. Perelló & C. A. Cordo

  2. Cerealicultura, Univ. Nacional de La Plata, Buenos Aires, Argentina

    M. R. Simón

  3. Inst. Spegazzini, Fac. de Ciencias Naturales y Museo, Univ. Nacional de La Plata, Buenos Aires, Argentina

    A. M. Arambarri

  4. Consejo Nacional de Investigaciones Científicas y Técnias, La Plata, Buenos Aires, Argentina

    C. A. Cordo

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  1. A. Perelló
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  4. C. A. Cordo
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Correspondence to A. Perelló.

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Perelló, A., Simón, M.R., Arambarri, A.M. et al. Greenhouse screening of the saprophytic resident microflora for control of leaf spots of wheat (Triticum aestivum). Phytoparasitica 29, 341–351 (2001). https://doi.org/10.1007/BF02981848

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  • DOI: https://doi.org/10.1007/BF02981848

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